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Simplifying quantitative measurement of free radical species using an X-band EPR spectrometer
https://repo.qst.go.jp/records/84974
https://repo.qst.go.jp/records/84974cc7a7939-08a0-4ea8-a9b0-8dd887ce9574
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Makino.Y.2022.J.Clin.Biochem.Nutr.70(3).213-221.pdf (801.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2021-06-24 | |||||
| タイトル | ||||||
| タイトル | Simplifying quantitative measurement of free radical species using an X-band EPR spectrometer | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Yusuke, Makino
× Yusuke, Makino× Megumi, Ueno× Yoshimi, Shoji× Minako, Nyui× Ikuo, Nakanishi× Fukui, Koji× Matsumoto, Ken-ichiro× Yusuke, Makino× Megumi, Ueno× Yoshimi, Shoji× Minako, Nyui× Ikuo, Nakanishi× Kenichiro, Matsumoto |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The quantitative measurement of free radical species in liquid using an X-band electron paramagnetic resonance (EPR) spectrometer was systematized. The quantitative ability of EPR spectrometers is principally poor. Therefore, quantification of free radical species by EPR requires a standard sample that contains a known spin amount/concentration. When satisfactory reproducibility of the sample material, volume, shape, and positioning in the cavity for all EPR measurements can be guaranteed, a sample tested and a standard can be directly compared and the process of comparisons for quantification can be simplified. The purpose of this study was to simplify manual quantitative EPR measurement. A suitable sample volume for achieving a stable X-band EPR signal intensity was estimated. The effects of different solvents on the EPR sensitivity were compared. The stability and reproducibility of the signal intensity of standard sample solutions of nitroxyl radicals were compared among different types of sample tubes. When the sample tubes, sample volumes, and/or solvents were the same, the EPR signal intensity was reproduced with an error of 2% or less for μM samples. Therefore, using the standard sample in the same solvent and the same volume drawn into the same sample tube, the EPR signal intensity of quantified samples as able to be directly compared with that of the standard sample. The standard sample for quantification should be measured just before or after every daily experiment. |
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| 書誌情報 |
Journal of Clinical Biochemistry and Nutrition 巻 70, 号 3, p. 213-221, 発行日 2021-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0912-0009 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3164/jcbn.21-83 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.3164/jcbn.21-83 | |||||
