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X-ray induced luminescence spectroscopy for DNA damaging intermediates aided by
https://repo.qst.go.jp/records/84971
https://repo.qst.go.jp/records/84971f2dab81d-21c1-4d73-a2ab-c13381826e66
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-08-06 | |||||
タイトル | ||||||
タイトル | X-ray induced luminescence spectroscopy for DNA damaging intermediates aided by | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Terao, Yusaku
× Terao, Yusaku× Kumagai, Yoshiaki× Suzuki, Issei× Tsuchiya, Takahiro× Ukai, Masatoshi× Akinari, Yokoya× Fujii, Kentaro× Akinari, Yokoya× Kentaro, Fujii |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Purpose: To identify the bonding sites of initial radiation interaction with DNA and to trace the following chemical reaction sequences on the pathway of damage induction, we carry out a spectroscopy XIL (X-ray induced luminescence) using soft X-ray synchrotron radiation. This is a non-destructive analysis of the excited intermediate species produced in a molecular mechanism on the damage induction pathway. Materials and Methods: We introduce aqueous samples of UMP (uridine-5’-monophosphate) in the vacuum by the use of a liquid micro-jet technique. The luminescence in the region of UV-VIS (from visible to ultraviolet) radiation induced after the absorption of monochromatic soft X-ray by aqueous UMP is measured with sweeping the soft X-ray energy in the region of 370 eV – 560 eV. Results: The enhanced XIL intensities for aqueous UMP in the region of soft X-ray of 410 eV – 530 eV (in “water window” region) are obtained (Kojima et al, 2019). The enhancement of XIL intensities in the UV-VIS region, relative to the water control, is explained by the excitation and ionization of a K-shell electron of nitrogen atoms in the uracil moiety. The enhanced XIL intensities do not match the structure of XANES (X-ray absorption near-edge structure) of the aqueous UMP. This suggests that the XIL intensities reflect the quantum yields of luminescence, or the quantum yields conversion by UMP of an absorbed X-ray into UV-VIS radiation. In this paper, spectra of luminescence are shown to be resolved by combining low pass filters. The filtered luminescence spectra are obtained at the center of gravity (λc) of the band pass wavelength regions at λc =270nm, 295 nm, 340nm, 385 nm, 450 nm, and 525 nm., which show a trend similar to the fluorescence of nucleobases induced by ultraviolet radiation. Conclusion: It is concluded that the origin of the observed XIL is the hydrated uracil moiety in aqueous UMP, decomposition of which is suppressed by the migration of excess charge and internal energy after the double ionization due to Auger decay. |
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書誌情報 |
International Journal of Radiation Biology 発行日 2021-08 |
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出版者 | ||||||
出版者 | Taylor & Francis | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0955-3002 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1080/09553002.2021.1967506 | |||||
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識別子タイプ | URI | |||||
関連識別子 | https://www.tandfonline.com/doi/full/10.1080/09553002.2021.1967506 |