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DNA strand break induction of aqueous plasmid DNA exposed to 30 MeV protons at ultra-high dose rate.
https://repo.qst.go.jp/records/84969
https://repo.qst.go.jp/records/8496920c29cc0-aa53-4a7e-a035-5929f35134d4
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-09-08 | |||||
| タイトル | ||||||
| タイトル | DNA strand break induction of aqueous plasmid DNA exposed to 30 MeV protons at ultra-high dose rate. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ohsawa, Daisuke
× Ohsawa, Daisuke× Hiroyama, Yota× Kobayashi, Alisa× Kitamura, HIsashi× Hojo, Satoru× Kusumoto, Tamon× Kodaira, Satoshi× Konishi, Teruaki× Daisuke, Ohsawa× Yota, Hiroyama× Alisa, Kobayashi× Hisashi, Kitamura× Satoru, Hojo× Tamon, Kusumoto× Satoshi, Kodaira× Teruaki, Konishi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Radiation cancer therapy with ultra-high dose rate exposure, so called FLASH radiotherapy, appears to reduce normal tissue damage without compromising tumor response. The aim of this study was to clarify whether FLASH exposure of proton beam would be effective in reducing the DNA strand break induction. We applied a simple model system, pBR322 plasmid DNA in aqueous 1× TE solution, where DNA single strand breaks (SSBs) and double strand breaks (DSBs) can be precisely quantified by gel electrophoresis. Plasmid DNA were exposed to 27.5 MeV protons in the conventional dose rate of 0.05 Gy/s (CONV) and ultra-high dose rate of 40 Gy/s (FLASH). With both dose rate, the kinetics of the SSB and DSB induction were proportional to absorbed dose. The SSB induction of FLASH was significantly less than CONV, which were 8.79 ± 0.14 (10-3 SSB per Gy per molecule) and 10.8 ± 0.68 (10-3 SSB per Gy per molecule), respectively. The DSB induction of FLASH was also slightly less than CONV, but difference was not significant. Altogether, 30 MeV proton beam at 40 Gy/s reduced SSB and not DSB, thus its effect may not be significant in reducing lethal DNA damage that become apparent in acute radiation effect. |
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| 書誌情報 |
Journal of Radiation Research 巻 63, 号 2, p. 255-260, 発行日 2021-12 |
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| 出版者 | ||||||
| 出版者 | Oxford University Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1093/jrr/rrab114 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1093/jrr/rrab114 | |||||
