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Molecular profiling of extracellular vesicles via charge-based capture using oxide nanowire microfluidics
https://repo.qst.go.jp/records/84711
https://repo.qst.go.jp/records/84711180b0447-363f-474c-83c0-898a26e82def
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-11-13 | |||||
| タイトル | ||||||
| タイトル | Molecular profiling of extracellular vesicles via charge-based capture using oxide nanowire microfluidics | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yasui, Takao
× Yasui, Takao× Paisrisarn, Piyawan× Yanagida, Takeshi× Konakade, Yuki× Nakamura, Yuta× Nagashima, Kazuki× Musa, Marina× Adiyasa Thiodorus, Ivan× Takahashi, Hiromi× Naganawa, Tsuyoshi× Shimada, Taisuke× Kaji, Noritada× Ochiya, Takahiro× Kawai, Tomoji× Baba, Yoshinobu× Yoshinobu, Baba |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Extracellular vesicles (EVs) have shown promising features as biomarkers for early cancer diagnoses. The outer layer of cancer cell-derived EVs consists of organotropic metastasis-induced membrane proteins and specifically enriched proteoglycans, and these molecular compositions determine EV surface charge. Although many efforts have been devoted to investigating the correlation between EV subsets obtained through density-, size-, and immunoaffinity-based captures and expressed membrane proteins, understanding the correlation between EV subsets obtained through surface charge-based capture and expressed membrane proteins is lacking. Here, we propose a methodology to profile membrane proteins of EV subsets obtained through surface charge-based capture. Nanowire-induced charge-based capture of EVs and in-situ profiling of EV membrane proteins are the two key methodology points. The oxide nanowires allowed EVs to be obtained through surface charge-based capture due to the diverse isoelectric points of the oxides and the large surface-to-volume ratios of the nanowire structures. And, with the ZnO nanowire device, whose use does not require any purification and concentration processes, we demonstrated the correlation between negatively-charged EV subsets and expressed membrane proteins derived from each cell. Furthermore, we determined that a colon cancer related membrane protein was overexpressed on negatively charged surface EVs derived from colon cancer cells. | |||||
| 書誌情報 |
Biosensors and Bioelectronics 巻 194, p. 113589, 発行日 2021-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0956-5663 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.bios.2021.113589 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.sciencedirect.com/science/article/pii/S0956566321006266 | |||||
