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Transiently structured head domains control intermediate filament assembly
https://repo.qst.go.jp/records/84699
https://repo.qst.go.jp/records/846999bba0115-945f-4201-b6e8-6159fc3fe33c
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-10-01 | |||||
| タイトル | ||||||
| タイトル | Transiently structured head domains control intermediate filament assembly | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Zhou, Xiaoming
× Zhou, Xiaoming× Lin, Yi× Masato, Kato× Mori, Eiichiro× Liszczak, Glen× Sutherland, Lillian× O. Sysoev, Vasiliy× T. Murray, Dylan× Tycko, Robert× L. McKnight, Steven× Masato, Kato |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Low complexity (LC) head domains 92 and 108 residues in length are, respectively, required for assembly of neurofilament light (NFL) and desmin intermediate filaments (IFs). As studied in isolation, these IF head domains interconvert between states of conformational disorder and labile, β-strand–enriched polymers. Solid-state NMR (ss-NMR) spectroscopic studies of NFL and desmin head domain polymers reveal spectral patterns consistent with structural order. A combination of intein chemistry and segmental isotope labeling allowed preparation of fully assembled NFL and desmin IFs that could also be studied by ss-NMR. Assembled IFs revealed spectra overlapping with those observed for β-strand–enriched polymers formed from the isolated NFL and desmin head domains. Phosphorylation and disease-causing mutations reciprocally alter NFL and desmin head domain self-association yet commonly impede IF assembly. These observations show how facultative structural assembly of LC domains via labile, β-strand–enriched self-interactions may broadly influence cell morphology. | |||||
| 書誌情報 |
Proceedings of the National Academy of Sciences of the United States of America 巻 118, 号 8, p. e2022121118, 発行日 2021-10 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0027-8424 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1073/pnas.2022121118 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.pnas.org/content/118/8/e2022121118 | |||||
