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Urinary MicroRNA-Based Diagnostic Model for Central Nervous System Tumors Using Nanowire Scaffolds
https://repo.qst.go.jp/records/84637
https://repo.qst.go.jp/records/846373d194ff2-1858-466e-acb2-979f9f9f6200
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-09-29 | |||||
| タイトル | ||||||
| タイトル | Urinary MicroRNA-Based Diagnostic Model for Central Nervous System Tumors Using Nanowire Scaffolds | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kitano, Yotaro
× Kitano, Yotaro× Aoki, Kosuke× Ohka, Fumiharu× Yamazaki, Shintaro× Motomura , Kazuya× Tanahashi, Kuniaki× Hirano, Masaki× Naganawa, Tsuyoshi× Iida, Mikiko× Shiraki, Yukihiro× Nishikawa, Tomohide× Shimizu, Hiroyuki× Yamaguchi, Junya× Maeda, Sachi× Suzuki, Hidenori× Wakabayashi, Toshihiko× Yoshinobu, Baba× Yasui, Takao× Natsume, Atsushi× Suzuki, Hidenori× Yoshinobu, Baba |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | There are no accurate mass screening methods for early detection of central nervous system (CNS) tumors. Recently, liquid biopsy has received a lot of attention for less-invasive cancer screening. Unlike other cancers, CNS tumors require efforts to find biomarkers due to the blood–brain barrier, which restricts molecular exchange between the parenchyma and blood. Additionally, because a satisfactory way to collect urinary biomarkers is lacking, urine-based liquid biopsy has not been fully investigated despite the fact that it has some advantages compared to blood or cerebrospinal fluid-based biopsy. Here, we have developed a mass-producible and sterilizable nanowire-based device that can extract urinary microRNAs efficiently. Urinary microRNAs from patients with CNS tumors (n = 119) and noncancer individuals (n = 100) were analyzed using a microarray to yield comprehensive microRNA expression profiles. To clarify the origin of urinary microRNAs of patients with CNS tumors, glioblastoma organoids were generated. Glioblastoma organoid-derived differentially expressed microRNAs (DEMs) included 73.4% of the DEMs in urine of patients with parental tumors but included only 3.9% of those in urine of noncancer individuals, which suggested that many CNS tumor-derived microRNAs could be identified in urine directly. We constructed the diagnostic model based on the expression of the selected microRNAs and found that it was able to differentiate patients and noncancer individuals at a sensitivity and specificity of 100 and 97%, respectively, in an independent dataset. Our findings demonstrate that urinary microRNAs extracted with the nanowire device offer a well-fitted strategy for mass screening of CNS tumors. | |||||
| 書誌情報 |
ACS Applied Materials & Interfaces 巻 13, 号 15, p. 17316-17329, 発行日 2021-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1944-8244 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1021/acsami.1c01754 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://pubs.acs.org/doi/abs/10.1021/acsami.1c01754 | |||||
