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Differential pial and penetrating arterial responses examined by optogenetic activation of astrocytes and neurons
https://repo.qst.go.jp/records/84594
https://repo.qst.go.jp/records/84594fdd2fbf6-86e4-4996-953f-037318bb52d1
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-10-07 | |||||
| タイトル | ||||||
| タイトル | Differential pial and penetrating arterial responses examined by optogenetic activation of astrocytes and neurons | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hatakeyama, Nao
× Hatakeyama, Nao× Miyuki, Unekawa× Juri Murata× Yutaka, Tomita× Norihiro Suzuki× Nakahara, Jin× Hiroyuki, Takuwa× Iwao, Kanno× Matsu, Ko× F Tanaka, Kenji× Kazuto, Masamoto× Miyuki, Unekawa× Yutaka, Tomita× Hiroyuki, Takuwa× Iwao, Kanno× Kazuto, Masamoto |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A variety of brain cells participates in neurovascular coupling by transmitting and modulating vasoactive signals. The present study aimed to probe cell type-dependent cerebrovascular (i.e., pial and penetrating arterial) responses with optogenetics in the cortex of anesthetized mice. Two lines of the transgenic mice expressing a step function type of light-gated cation channel (channelrhodopsine-2; ChR2) in either cortical neurons (muscarinic acetylcholine receptors) or astrocytes (Mlc1-positive) were used in the experiments. Photo-activation of ChR2-expressing astrocytes resulted in a widespread increase in cerebral blood flow (CBF), extending to the nonstimulated periphery. In contrast, photo-activation of ChR2-expressing neurons led to a relatively localized increase in CBF. The differences in the spatial extent of the CBF responses are potentially explained by differences in the involvement of the vascular compartments. In vivo imaging of the cerebrovascular responses revealed that ChR2-expressing astrocyte activation led to the dilation of both pial and penetrating arteries, whereas ChR2-expressing neuron activation predominantly caused dilation of the penetrating arterioles. Pharmacological studies showed that cell type-specific signaling mechanisms participate in the optogenetically induced cerebrovascular responses. In conclusion, pial and penetrating arterial vasodilation were differentially evoked by ChR2-expressing astrocytes and neurons. | |||||
| 書誌情報 |
Journal of Cerebral Blood Flow & Metabolism 巻 41, 号 10, p. 2676-2689, 発行日 2021-04 |
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| 出版者 | ||||||
| 出版者 | SAGE | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0271-678X | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 33899558 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1177/0271678X211010355 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://journals.sagepub.com/doi/full/10.1177/0271678X211010355 | |||||
