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Development of mutant microalgae that accumulate lipids under nitrate-replete conditions
https://repo.qst.go.jp/records/84567
https://repo.qst.go.jp/records/8456723d4aa83-659e-43df-b5fa-5458e02737da
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-08-31 | |||||
| タイトル | ||||||
| タイトル | Development of mutant microalgae that accumulate lipids under nitrate-replete conditions | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Oyama, Tomoki
× Oyama, Tomoki× Kato, Yuichi× Sato, Katsuya× Ono, Yutaka× Matsuda, Mami× Hasunuma, Tomohisa× Kondo, Akihiko× Katsuya, Sato× Yutaka, Ono |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Microalgal biofuels have attracted global attention as an alternative energy source to fossil fuels. Microalgae generally accumulate triacylglycerols (lipids) under nitrogen-deficient conditions; however, cell growth is highly suppressed under these conditions, subsequently reducing lipid productivity. Therefore, microalgae that can accumulate lipids even under nitrogen-replete conditions are needed to realize the one-step nutrient-replete cultivation with improved lipid productivity. Thus, the aim of this study was to develop a method for screening mutant microalgae showing high lipid accumulation in the presence of a nitrogen source. Mutant cells were generated by irradiating the oleaginous green microalga Chlamydomonas sp. KOR1 with carbon-ion beams, followed by cultivation under nitrate-replete conditions, and then subjected to fluorescence-activated cell sorting-based screening for detecting lipid-rich cells. By repeatedly performing the sequential procedures of cultivation and selection, the strains KAC1710 and KAC1801, which showed high lipid accumulation under nitrate-replete conditions, were obtained. In the presence of abundant nitrate, these mutants formed a large number of lipid droplets in the cells, and achieved 1.5-fold (KAC1710) and 2.1-fold (KAC1801) higher lipid content than KOR1. Metabolome analysis revealed that the pool size of pyruvate was significantly increased in KAC1710 and KAC1801, consistent with the activation of lipid synthesis under nitrate-replete conditions. In conclusion, this study reports the use of a novel nitrogen-based screening method for developing mutant microalgae that accumulate lipids in the presence of a nitrogen source, and found that an increase in pyruvate pool size is an important metabolic phenomenon associated with lipid production. | |||||
| 書誌情報 |
Algal Research 巻 60, p. 102544, 発行日 2021-12 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2211-9264 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.algal.2021.102544 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.sciencedirect.com/science/article/abs/pii/S2211926421003635 | |||||
