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Expression of an X-Ray Irradiated EGFP-Expressing Plasmid Transfected into Nonirradiated Human Cells
https://repo.qst.go.jp/records/83415
https://repo.qst.go.jp/records/834154e78c47c-8e5a-4cdf-b829-58e4d9a178e2
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-09-11 | |||||
| タイトル | ||||||
| タイトル | Expression of an X-Ray Irradiated EGFP-Expressing Plasmid Transfected into Nonirradiated Human Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yui, Obata
× Yui, Obata× Obata, Yui× Nakaue, Hiroaki× Keishiro, Hirasaki× Hirasaki, Keishiro× Akimitsu, Nobuyoshi× Akinari, Yokoya× Yui, Obata× Keishiro, Hirasaki× Akinari, Yokoya |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To investigate the repairability of X-ray-induced DNA damage, particularly non-double-strand breaks in living cells, enhanced green fluorescent protein (EGFP)-expressing plasmids were exposed to X-rays and then transfected into non-irradiated human cells, MCF7 and MCF10A. Live-cell imaging of EGFP fluorescence was performed to measure the efficiency of plasmid repair in cells. The number of EGFP-expressing cells significantly decreased with increasing X-ray dose for both cell lines. The obtained kinetic curves of EGFP expression indicating plasmid repair were quantitatively compared with algebraically calculated ones based on the values of the transfected plasmids that had been treated with nicking or restriction enzymes. Then, assuming a Poisson distribution of SSBs, the number of cells carrying these nicked plasmids that could express EGFP were estimated. Our experimental results revealed considerably fewer cells expressing EGFP compared with the expected values we had calculated. These results suggest that the lower proportion of cells expressing EGFP as a measure of plasmid repair was due not only to the complex chemical structures of termini created by single-strand breaks compared with those created by enzyme treatments, but also that base lesions or AP sites proximately arising at the strand break termini might compromise EGFP expression. These results emphasize that radiation-induced DNA breaks are less repairable than enzymatically induced ones, which is not apparent when using conventional gel electrophoresis assays of plasmid DNA. | |||||
| 書誌情報 |
Radiation Research 巻 196, p. 261-271, 発行日 2021-07 |
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| 出版者 | ||||||
| 出版者 | Radiation Research Society (US) | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1667/RR15399.1 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://meridian.allenpress.com/radiation-research/article-abstract/196/3/261/467621/Expression-of-an-X-Ray-Irradiated-EGFP-Expressing?redirectedFrom=fulltext | |||||
