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Dynamical differences between polymorphs of lysozyme amyloid fibrils with different levels of cytotoxicity
https://repo.qst.go.jp/records/82966
https://repo.qst.go.jp/records/82966e39e5406-d43c-4547-b74b-43c036d34d8d
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-05-19 | |||||
| タイトル | ||||||
| タイトル | Dynamical differences between polymorphs of lysozyme amyloid fibrils with different levels of cytotoxicity | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Tatsuhito, Matsuo
× Tatsuhito, Matsuo× Peters, Judith× Tatsuhito, Matsuo |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Amyloid fibrils are self-assembled protein filaments, the deposition of which in tissues causes amyloidosis. Recently, much attention has been paid to polymorphism, where proteins form various amyloid fibrils that differ in structure and show different levels of cytotoxicity depending on fibrillation conditions. Since intramolecular motions in the fibrils are considered to play a crucial role in amyloidosis, it is important to understand the dynamical features of their polymorphs. In this study, we focused on the polymorphism of hen egg white lysozyme ( HEWL), a model for studying lysozyme amyloidosis in human and prepared D2O hydrated powder samples of two HEWL amyloid polymorphs formed at pH6 or 2, which are known to show distinct levels of cytotoxicity. We carried out the elastic incoherent neutron scattering (EINS) measurements on these samples using the IN13 spectrometer at ILL in France in the temperature range from 20 K to 310 K. The temperature dependences of the mean square displacements (MSDs) of atomic motions in the proteins were evaluated from the EINS spectra. It was found that whereas the MSD values are similar between the two fibrils at lower temperatures, the fibrils with higher toxicity show significantly larger MSDs at higher temperatures (>> 270 K). This implies that the differences in the anharmonic, diffusive local motions are related to the differences in the level of cytotoxicity. At the conference, more detailed results will be presented. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | MLZ Conference 2021: Neutrons for Life Sciences | |||||
| 発表年月日 | ||||||
| 日付 | 2021-06-09 | |||||
| 日付タイプ | Issued | |||||
