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Fe-S cluster binding mechanism of pigeon's ISCA1 clarified by SEC-SAXS
https://repo.qst.go.jp/records/82906
https://repo.qst.go.jp/records/829064e3e8d6b-08d0-4922-a180-f6cc2859a2fc
| Item type | 会議発表論文 / Conference Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-05-21 | |||||
| タイトル | ||||||
| タイトル | Fe-S cluster binding mechanism of pigeon's ISCA1 clarified by SEC-SAXS | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||
| 資源タイプ | conference paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Shigeki, Arai
× Shigeki, Arai× Rumi, Shimizu× Motoyasu, Adachi× Mitsuhiro, Hirai× Shigeki, Arai× Rumi, Shimizu× Motoyasu, Adachi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The iron-sulfur cluster assembly 1 homolog clISCA1 of Columba livia (pigeon) interacts with a quantum biosensor protein clCRY4. The clCRY4/clISCA1 complex tends to orientate along the weak external magnetic-field lines (0.4–10G) under blue light, suggesting that clISCA1 might assist the function of clCRY4. It was expected that the Fe-S cluster binging to clISCA1 might improve the magnetic property of clISCA1. In order to elucidate the Fe-S cluster binging mechanism of clISCA1, we conducted the small angle X-ray scattering analysis coupled with size exclusion chromatography analysis (SEC-SAXS) and UV/Vis spectroscopy. The result suggests that the globular protomers of clISCA1 form columnar oligomers, and Fe-S cluster binding sites are formed between clISCA1 protomers in a columnar oligomer. Periodic and regular binding of Fe-S clusters along the long axis of the columnar oligomer may improve the magnetic susceptibility and the magnetic anisotropy of the clISCA1 oligomer and the clCRY4/clISCA1 complex. | |||||
| 書誌情報 |
Photon Factory Activity Report 巻 2021, 号 38, p. 60, 発行日 2021-06 |
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| 出版者 | ||||||
| 出版者 | 高エネルギー加速器研究機構 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://pfwww.kek.jp/acr/2020pdf/u_reports/pf20b0060.pdf | |||||
