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Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching enzyme
https://repo.qst.go.jp/records/82752
https://repo.qst.go.jp/records/82752f7347ee1-78a3-4be3-92a2-91f3757b61a4
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-08-31 | |||||
| タイトル | ||||||
| タイトル | Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching enzyme | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kato, Yuichi
× Kato, Yuichi× Oyama, Tomoki× Inokuma, Kentaro× J. Vavricka, Christopher× Matsuda, Mami× Hidese, Ryota× Sato, Katsuya× Ono, Yutaka× Jo-Shu, Chang× Hasunuma, Tomohisa× Kondo, Akihiko× Katsuya, Sato× Yutaka, Ono |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Light/dark cycling is an inherent condition of outdoor microalgae cultivation, but is often unfavorable for lipid accumulation. This study aims to identify promising targets for metabolic engineering of improved lipid accumulation under outdoor conditions. Consequently, the lipid-rich mutant Chlamydomonas sp. KOR1 was developed through light/dark-conditioned screening. During dark periods with depressed CO2 fixation, KOR1 shows rapid carbohydrate degradation together with increased lipid and carotenoid contents. KOR1 was subsequently characterized with extensive mutation of the ISA1 gene encoding a starch debranching enzyme (DBE). Dynamic time-course profiling and metabolomics reveal dramatic changes in KOR1 metabolism throughout light/dark cycles. During light periods, increased flux from CO2 through glycolytic intermediates is directly observed to accompany enhanced formation of small starch-like particles, which are then efficiently repartitioned in the next dark cycle. This study demonstrates that disruption of DBE can improve biofuel production under light/dark conditions, through accelerated carbohydrate repartitioning into lipid and carotenoid. | |||||
| 書誌情報 |
Communications Biology 巻 4, p. 450, 発行日 2021-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2399-3642 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1038/s42003-021-01976-8 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.nature.com/articles/s42003-021-01976-8 | |||||
