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蛍光ナノダイヤモンドによる幹細胞再生機能温度センシング
https://repo.qst.go.jp/records/82673
https://repo.qst.go.jp/records/8267350255fb1-7df6-4116-a8e6-1f8ef7f97713
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-04-08 | |||||
| タイトル | ||||||
| タイトル | 蛍光ナノダイヤモンドによる幹細胞再生機能温度センシング | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
宮地, 冬
× 宮地, 冬× 小林, 香央里× 西村, 勇姿× 藤原, 正澄× 湯川, 博× 馬場, 嘉信× Yushi, Nishimura× Hiroshi, Yukawa× Yoshinobu, Baba |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | It has become clear that temperature plays a major role in many life phenomena. However, little is known about the intracellular temperature, and the relationship between regenerative function and intracellular temperature of stem cells. In this study, fluorescent nanodiamond (FND) that can be applied as an excellent quantum sensor was used for stem cell labeling. Then, the relationship between the regenerative function of stem cells and the intracellular temperature of stem cells was evaluated by a sensing the optically detected magnetic resonance (ODMR) activity of NV centers in FNDs. FNDs could label adipose tissue-derived stem cells (ASCs) at high efficiency with 24 h incubation, and no cytotoxicity was observed in ASCs labeled with less than 500 µg/mL of FNDs. The ODMR of NV centers in FNDs was confirmed at approximately 2.87 GHz with a characteristic fluorescence spectrum, and the intracellular temperature of living ASCs during several days of culturing could be precisely measured. Moreover, the intracellular temperature was found to influence the production of growth factors and the degree of differentiation into adipocytes and osteocytes. These data suggest that NV centers in FNDs can be used to investigate the relationship between intracellular temperature and cellular functions. | |||||
| 書誌情報 |
Organ Biology 巻 27, 号 2, p. 185-190, 発行日 2020-08 |
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| 出版者 | ||||||
| 出版者 | 日本臓器保存生物医学会 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1340-5152 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.11378/organbio.27.185 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.jstage.jst.go.jp/article/organbio/27/2/27_185/_article/-char/ja/ | |||||
