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Real-time nanodiamond thermometry probing in vivo thermogenic responses
https://repo.qst.go.jp/records/82668
https://repo.qst.go.jp/records/82668d3095be8-d95b-4481-8b10-28cc3a2c2392
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-04-08 | |||||
| タイトル | ||||||
| タイトル | Real-time nanodiamond thermometry probing in vivo thermogenic responses | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fujiwara, Masazumi
× Fujiwara, Masazumi× Sun, Simo× Dohms, Alexander× Yushi, Nishimura× Suto, Ken× Takezawa, Yuka× Oshimi, Keisuke× Zhao, Li× Sadzak, Nikola× Umehara, Yumi× Teki, Yoshio× Komatsu, Naoki× Benson, Oliver× Shikano, Yutaka× Eriko, Kage-Nakadai× Yushi, Nishimura |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Real-time temperature monitoring inside living organisms provides a direct measure of their biological activities. However, it is challenging to reduce the size of biocompatible thermometers down to submicrometers, despite their potential applications for the thermal imaging of subtissue structures with single-cell resolution. Here, using quantum nanothermometers based on optically accessible electron spins in nanodiamonds, we demonstrate in vivo real-time temperature monitoring inside Caenorhabditis elegans worms. We developed a microscope system that integrates a quick-docking sample chamber, particle tracking, and an error correction filter for temperature monitoring of mobile nanodiamonds inside live adult worms with a precision of ±0.22°C. With this system, we determined temperature increases based on the worms’ thermogenic responses during the chemical stimuli of mitochondrial uncouplers. Our technique demonstrates the submicrometer localization of temperature information in living animals and direct identification of their pharmacological thermogenesis, which may allow for quantification of their biological activities based on temperature. | |||||
| 書誌情報 |
Science Advances 巻 6, 号 37, p. eaba9636, 発行日 2020-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2375-2548 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1126/sciadv.aba9636 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://advances.sciencemag.org/content/6/37/eaba9636 | |||||
