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Calcium imaging in freely behaving Caenorhabditis elegans with well-controlled, nonlocalized vibrations
https://repo.qst.go.jp/records/82658
https://repo.qst.go.jp/records/826580967e85c-97f9-4f88-be7b-6f921ab1f06e
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-04-07 | |||||
| タイトル | ||||||
| タイトル | Calcium imaging in freely behaving Caenorhabditis elegans with well-controlled, nonlocalized vibrations | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Shigyou, Kazuki
× Shigyou, Kazuki× Maeoka, Haruka× Igarashi, Ryuji× Sugi, Takuma× Ryuji, Igarashi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Nonlocalized mechanical forces, such as vibrations and acoustic waves, influence a wide variety of biological processes from development to homeostasis. Animals cope with these stimuli by modifying their behavior. Understanding the mechanisms underlying such behavioral modification requires quantification of neural activity during the behavior of interest. Here, we report a method for calcium imaging in freely behaving Caenorhabditis elegans with nonlocalized vibration of specific frequency, displacement, and duration. This method allows the production of well-controlled, nonlocalized vibration using an acoustic transducer and quantification of evoked calcium responses at single-cell resolution. As a proof of principle, the calcium response of a single interneuron, AVA, during the escape response of C. elegans to vibration is demonstrated. This system will facilitate understanding of neural mechanisms underlying behavioral responses to mechanical stimuli. | |||||
| 書誌情報 |
Journal of Visualized Experiments 巻 170, p. e61626, 発行日 2021-05 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1940-087X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3791/61626 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.jove.com/t/61626/calcium-imaging-freely-behaving-caenorhabditis-elegans-with-well | |||||
