WEKO3
アイテム
Damage and Growth Inhibition of Bacillus subtilis Spores by Ion-beam, Gamma Irradiation and Heat Treatment.
https://repo.qst.go.jp/records/82305
https://repo.qst.go.jp/records/823053b0a9169-0755-43da-b3e2-24e4cf5ebf64
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-03-19 | |||||
| タイトル | ||||||
| タイトル | Damage and Growth Inhibition of Bacillus subtilis Spores by Ion-beam, Gamma Irradiation and Heat Treatment. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Den, Hiroki
× Den, Hiroki× Futenma, Akira× Sakai, Toshio× Maeda, Nobuhiko× Sakamoto, Hitoshi× Tsuchido, Tetsuaki× Furuta, Masakazu× Katsuya, Sato× Yutaka, Ono× Katsuya, Sato× Yutaka, Ono |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Bacillus subtilis is known as Gram-positive, rod-shaped, aerobic, spore-forming bacterium that is naturally found in soil. Bacillus spores have a hard, layered structure depositing 2,6-Pyridinedicarboxylic acid (DPA) in their dehydrated core region containing genomic DNA protected by acid-soluble small proteins contributing the resistance of the spores against various bactericidal stresses. such as heat, desiccation, chemical, and radiation. The spores release DPA from their cores together with hydration during the germination process and vegetative cells are formed through subsequent outgrowth and moved to vegetative growth again. These processes can be monitored by tracking the turbidity changes of liquid culture because the refractive index of light scattering changes within the culture during spore germination. Besides, Ionizing radiation induces DNA damages including single-strand breaks and double-strand breaks and generates reactive oxygen species which also induces DNA strand breaks. In this work, we used B. subtilis 168 (trpC2) spores, and the germination and growth process of the spores was investigated by 60Co γ-rays and ion beam irradiation in comparison with heat treatment. The 12C6+ ion beam-irradiated spores showed the most sensitivity among the ion beams used for irradiations. The lethal effect of spore was estimated to be in the order 12C6+ > 20Ne8+ > 4He2+. The initial spore germination process ion–beam irradiated spores seemed to be similar with 60Co-sgamma rays. Outgrowth phase after the germination became longer with their irradiation sensitivity increased according to different LET of each ion beam species. These results suggest that damaged sites in spores are similar between gamma- and ion-beam irradiations. | |||||
| 書誌情報 |
QST Takasaki Annual Report 2019 巻 QST-M-29, p. 100-100, 発行日 2021-03 |
|||||
| 出版者 | ||||||
| 出版者 | QST | |||||
