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Nitrogen-Conditioned Screening of Chlamydomonas Mutants That Do Cell Growth and Lipid Accumulation Simultaneously Under Nitrate-Replete Condition
https://repo.qst.go.jp/records/82303
https://repo.qst.go.jp/records/823038da6518c-d25d-4c57-bb84-429f883e1b26
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-03-19 | |||||
| タイトル | ||||||
| タイトル | Nitrogen-Conditioned Screening of Chlamydomonas Mutants That Do Cell Growth and Lipid Accumulation Simultaneously Under Nitrate-Replete Condition | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Oyama, Tomoki
× Oyama, Tomoki× Kato, Yuichi× Hasunuma, Tomohisa× Ogino, Chiaki× Katsuya, Sato× Yutaka, Ono× Kondo, Akihiko× Katsuya, Sato× Yutaka, Ono |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Microalgae biofuel has attracted global attention as a new energy resource that replaces fossil fuels. Although there are valuable microalgal strains for biofuel production, complexity in cultivation process remains as the problem. Microalgae generally do cell growth by consuming nitrogen source, and accumulate lipids in the cells after completely consume nitrogen source. Thus, nitrogen deficiency is a common trigger for lipid accumulation in microalgae, however, it drastically inhibits cell growth. To avoid this trade-off problem between cell growth and lipid accumulation, this study aimed to develop a breeding method of microalgae strains that can simultaneously perform cell growth and lipid accumulation under nitrate-replete conditions. In this study, random mutagenesis by heavy ion beams was combined with nitrate-conditioned and fluorescence-activated cell sorter (FACS)-based screening. Then, the mutants were cultured under the nitrate-replete conditions, stained with lipophilic fluorescence dye BODIPY, and subjected to FACS screening. After repeating the sequential procedures to sort cells with high-BODIPY fluorescence, KAC1710, which can accumulate lipid under the nitrate-replete conditions, was obtained. The procedure was further repeated using KAC1710 as the parent. Consequently, KAC1801, which can accumulate more lipids than KAC1710 was obtained. KAC1710 and KAC1801 showed decreases in biomass production and nitrate consumption, while lipid content and lipid productivity under the nitrate-replete conditions were significantly improved in these mutants compared. These results suggest that restricted nitrate intake triggered direction of carbon flux to lipid synthesis. | |||||
| 書誌情報 |
QST Takasaki Annual Report 2019 巻 QST-M-29, p. 99-99, 発行日 2021-03 |
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| 出版者 | ||||||
| 出版者 | QST | |||||
