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Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior
https://repo.qst.go.jp/records/81955
https://repo.qst.go.jp/records/819558f925bc7-a3bf-4e71-9228-6d5f8b0edf94
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-02-23 | |||||
| タイトル | ||||||
| タイトル | Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Shino, Takeda
× Shino, Takeda× 藤代, 瞳× Izumi, Tanaka× Haruko, Yakumaru× Kyoko, Ayama× Akihiro, Uehara× Masakazu, Oikawa× 姫野, 誠一郎× Hiroshi, Ishihara× Shino, Takeda× Izumi, Tanaka× Haruko, Yakumaru× Kyoko, Ayama× Akihiro, Uehara× Masakazu, Oikawa× Hiroshi, Ishihara |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Nephrotoxicity is the primary health effect of uranium exposure. However, the renal transport and intracellular behavior of uranium remains to be clearly elucidated. In the present study, the intracellular uranium distribution was examined with the cell lines derived from the S3 segment of mouse renal proximal tubules, which is a toxic target site of uranium, using microbeambased elemental analysis. Uranium exposure at 100 μM for 24 h (non-toxic phase) was performed in S3 cells. Two types of measurement specimens, including those that are adhesive cell specimens and cryosection specimens, were examined for the positional relationship of the intracellular localization of uranium. Based on the combined results of single-cell imaging from the two types of cell specimens, uranium was distributed inside the cell and localized in the cytoplasm near the cell nucleus. In some cells, uranium was colocalized with phosphorus and potassium. The amount of uranium accumulated in S3 cells was estimated using thin section-standards. The mean uranium content of three adhesive cells was hundreds of femtogram per cell. Thus, we believe that single-cell imaging would be useful for studies on renal uranium transportation and cellular behavior. |
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| 書誌情報 |
Minerals 巻 11, 号 2, p. 191, 発行日 2021-02 |
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| 出版者 | ||||||
| 出版者 | MDPI | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2075-163X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3390/min11020191 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.3390/min11020191 | |||||
