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Expression analysis of genes associated with the radioprotective effect of p53 modulator 5CHQ on acute intestinal radiation injury
https://repo.qst.go.jp/records/81903
https://repo.qst.go.jp/records/819034a2783af-8938-4236-8f85-b27c7c43885e
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-02-19 | |||||
| タイトル | ||||||
| タイトル | Expression analysis of genes associated with the radioprotective effect of p53 modulator 5CHQ on acute intestinal radiation injury | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sakai, Takuma
× Sakai, Takuma× Akinori, Morita× Akinori Kanai× Wang, Bing× Enomoto, Atsushi× Ujita, Shohei× Aoki, Shin× Hidetoshi Sato× Sakaue, Masahiro× Nishiyama, Yuichi× Inaba, Toshiya× Takuma, Sakai× Akinori, Morita× Bing, Wang× Yuichi, Nishiyama× Toshiya, Inaba |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In radiation therapy, the presence of highly radiosensitive organs around the target tumor limits the prescription dose. To develop radioprotectors that are specific for normal tissues is a solution to increase the tolerance dose of these organs. We have previously reported that an 8-hydroxyquinoline derivative, 5-chloro-8-quinolinol (5CHQ), exerts a significant radioprotective effect in a p53-dependent manner. The characteristic action of 5CHQ is to upregulate a radioprotective p53 target gene, Cdkn1a, and to downregulate a proapoptotic p53 target gene, Bbc3. The purpose of this study is to discover new genes involved in the radioresistance by 5CHQ treatment other than Cdkn1a and Bbc3. In a comprehensive analysis of mRNA expression of the jejunal epithelium using female ICR mice, the gene expression changes of irradiated mice with or without 5CHQ were examined by mRNA-seq analysis using a next-generation sequencer. Based on the obtained results, absolute qPCR quantification was performed to confirm the reproducibility of mRNA expression changes. Expression of the well-known p53 target genes was not largely changed, but humoral factors involved in intestinal stem cell protection were increased by 5CHQ. Furthermore, by comparing Trp53-knockout mice with wild-type mice, the expression of some cytokines was found to be p53-dependent. We consider that some of these genes will be the candidate genes that are responsible for the radioprotection by 5CHQ. We plan to examine the radioprotective effects of these humoral candidate factors in the intestine to identify new protective factors in 5CHQ action. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 放射線災害・医科学拠点第５回国際シンポジウム | |||||
| 発表年月日 | ||||||
| 日付 | 2021-02-08 | |||||
| 日付タイプ | Issued | |||||
