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Torsional stress can regulate the unwrapping of two outer half superhelical turns of nucleosomal DNA
https://repo.qst.go.jp/records/81798
https://repo.qst.go.jp/records/817987227d8da-ce2f-412a-a946-45bf4f76c174
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-10-09 | |||||
| タイトル | ||||||
| タイトル | Torsional stress can regulate the unwrapping of two outer half superhelical turns of nucleosomal DNA | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ishida, Hisashi
× Ishida, Hisashi× Kono, Hidetoshi× Hisashi, Ishida× Hidetoshi, Kono |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Torsional stress has a significant impact on the structure and stability of the nucleosome. RNA polymerase imposes torsional stress on the DNA in chromatin and unwraps the DNA from the nucleosome to access the genetic information encoded in the DNA. To understand how the torsional stress affects the stability of the nucleosome, we examined the unwraping of the outer superhelical turn of the nucleosomal DNA under torsional stress with all-atom molecular dynamics simulations. The free energies for unwrapping the DNA indicate that positive stress that overtwists DNA facilitates asymmetric unwrapping of the DNA while negative stress that undertwists DNA does not. Under positive stress, DNA was straightened and rigid, which facilitated a large-scale asymmetric unwrapping of the DNA without a large extension of the DNA in which one end of the DNA was disconnected from H3 and H2A/H2B while the other end of the DNA stably remained wrapped. Under negative stress, the DNA became more bendable and flexible, which facilitated the binding of the unwrapped DNA to the octamer in a stable state. Consequently, we conclude that the torsion stress has a significant impact on the affinity of the DNA and the octamer through the inherent dynamic nature of the DNA: bendability and flexibility and can drastically change chromatin structure. | |||||
| 書誌情報 |
Proceedings of the National Academy of Sciences of the United States of America 巻 118, 号 7, p. e2020452118, 発行日 2021-02 |
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| 出版者 | ||||||
| 出版者 | PNAS | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0027-8424 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1073/pnas.2020452118 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.pnas.org/content/118/7/e2020452118 | |||||
