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X-ray induced cell cycle arrest and raises the cellular temperature
https://repo.qst.go.jp/records/81422
https://repo.qst.go.jp/records/81422eb812836-b131-4d23-a19a-b60d817efe6a
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-12-18 | |||||
| タイトル | ||||||
| タイトル | X-ray induced cell cycle arrest and raises the cellular temperature | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ihara, Tomokazu
× Ihara, Tomokazu× Noguchi, Miho× 岡部, 弘基× Yokoya, Akinari× Ihara, Tomokazu× Noguchi, Miho× Yokoya, Akinari |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Introduction Mammalian fibroblast cells undergo cellular senescence by high dose X-ray irradiation. Although the senescence is distinct from those caused by telomere shortening, both exhibit similar characteristic such as being irreversible cell cycle arrest and releasing bioactive substances that promote carcinogenesis or induce further senescence in surrounding cells. The evidence suggests that intracellular physiological reaction of senescent cells might be different from those of normal cells. We focused on intracellular temperature, as one of physicochemical index to evaluate the physiological changes in senescent cells. ATP molecules consumed in various reactions transfer energy to conjugated reactions, and then some of the energy might be converted to heat which possibly raises intracellular temperature. To clarify whether cellular temperature correlated with radiation-induced senescence, we performed measurements of the cellular temperature by introducing a thermoprobe, Cellular Thermoprobe for Fluorescence Ratio, into immortalized human fibroblast cells. Method The cells were exposed to 20 Gy X-rays. More than 90% of cells were confirmed undergoing senescence on eighth day after irradiation by SA-β-gal staining method. The thrmoprobe was introduced into the cultured cells, and then the ratio of red and green fluorescence intensity was determined with microscopic fluorescent images. Intracellular ATP levels was also measured by a luciferin-luciferase method. Result and Discussion The temperature of senescent cells was about 4°C higher than that of normal cells. This suggest that heat release reaction may be increasing in senescent cells. We also found a higher level of intracellular ATP in senescent cells than that in normal cells. This may be due to reduced ATP consumption with the arrest of the cell cycle. From these observations, it is inferred that deconjugation in mitochondria in senescent cells results in temperature evaluation without any ATP consumption. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 3rd International Forum on Quantum Metrology and Sensing | |||||
| 発表年月日 | ||||||
| 日付 | 2020-12-17 | |||||
| 日付タイプ | Issued | |||||
