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Bundling of mRNA strands inside polyion complexes improves mRNA delivery efficiency in vitro and in vivo.
https://repo.qst.go.jp/records/81400
https://repo.qst.go.jp/records/8140096d619c8-2418-4ab3-a06f-bd647730fd76
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-12-22 | |||||
| タイトル | ||||||
| タイトル | Bundling of mRNA strands inside polyion complexes improves mRNA delivery efficiency in vitro and in vivo. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Koji, Kyoko
× Koji, Kyoko× Yoshinaga, Naoto× Mochida, Yuki× Hong, Taehun× Miyazaki, Takuya× Kataoka, Kazunori× Osada, Kensuke× Cabral, Horacio× Uchida, Satoshi× Kensuke, Osada |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | RNA nanotechnology has promise for developing mRNA carriers with enhanced physicochemical and functional properties. However, the potential synergy for mRNA delivery of RNA nanotechnology in cooperation with established carrier systems remains unknown. This study proposes a combinational system of RNA nanotechnology and mRNA polyplexes, by focusing on mRNA steric structure inside the polyplexes. Firstly, several mRNA strands are bundled through hybridization with RNA oligonucleotide crosslinkers to obtain tight mRNA structure, and then the bundled mRNA is mixed with poly(ethylene glycol) (PEG)-polycation block copolymers to prepare PEG-coated polyplex micelles (PMs). mRNA bundling results in highly condensed mRNA packaging inside PM core with dense PEG chains on the surface, thereby, improving PM stability against polyion exchange reaction and ribonuclease (RNase) attack. Importantly, such stabilization effects are attributed to bundled structure of mRNA rather than the increase in total mRNA amount encapsulated in the PMs, as encapsulation of long mRNA strands without bundling fails to improve PM stability. Consequently, PMs loading bundled mRNA exhibit enhanced stability in mouse blood circulation, and induce efficient protein expression in cultured cells and mouse brain. | |||||
| 書誌情報 |
Biomaterials 巻 261, p. 120332, 発行日 2020-12 |
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| 出版者 | ||||||
| 出版者 | Elsevier Science | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0142-9612 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 32877764 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.biomaterials.2020.120332 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.sciencedirect.com/science/article/abs/pii/S0142961220305780 | |||||
