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Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice
https://repo.qst.go.jp/records/80912
https://repo.qst.go.jp/records/809126277f967-9404-4c32-ba69-14f98602f279
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-11-06 | |||||
| タイトル | ||||||
| タイトル | Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Liu, Cuihua
× Liu, Cuihua× Tanaka, Kaoru× Katsube, Takanori× Vares, Guillaume× Maruyama, Kouichi× Ninomiya, Yasuharu× Fardous, Zeenath× Sun, Chao× Fujimori, Akira× G. Moreno, Stephanie× Nenoi, Mitsuru× Wang, Bing× Liu, Cuihua× Kaoru, Tanaka× Takanori, Katsube× Vares, Guillaume× Kouichi, Maruyama× Yasuharu, Ninomiya× Fardous, Zeenath× Sun, Chao× Akira, Fujimori× Mitsuru, Nenoi× Bing, Wang |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Application of green fluorescent protein (GFP) in a variety of biosystems as a unique bioindicator or biomarker has revolutionized biological research and made groundbreaking achievements, while increasing evidence has shown alterations in biological properties and physiological functions of the cells and animals overexpressing transgenic GFP. In this work, response to total body irradiation (TBI) was comparatively studied in GFP transgenic C57BL/6-Tg (CAG-EGFP) mice and C57BL/6 N wild type mice. It was demonstrated that GFP transgenic mice were more sensitive to radiation-induced bone marrow death, and no adaptive response could be induced. In the nucleated bone marrow cells of GFP transgenic mice exposed to a middle dose, there was a significant increase in both the percentage of cells expressing pro-apoptotic gene Bax and apoptotic cell death. While in wild type cells, lower expression of pro-apoptotic gene Bax and higher expression of anti-apoptotic gene Bcl-2, and significant lower induction of apoptosis were observed compared to GFP transgenic cells. Results suggest that presence of GFP could alter response to TBI at whole body, cellular and molecular levels in mice. These findings indicate that there could be a major influence on the interpretation of the results obtained in GFP transgenic mice. | |||||
| 書誌情報 |
Dose-Response 巻 18, 号 3, p. 1559325820951332, 発行日 2020-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1559-3258 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1177/1559325820951332 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://journals.sagepub.com/doi/full/10.1177/1559325820951332 | |||||
