WEKO3
アイテム
Effects of Arabidopsis Ku80 deletion on the integration of the left border of T-DNA into plant chromosomal DNA via Agrobacterium tumefaciens
https://repo.qst.go.jp/records/80824
https://repo.qst.go.jp/records/80824a1dd6041-438a-4f07-9cda-f6352992d35d
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-04-06 | |||||
| タイトル | ||||||
| タイトル | Effects of Arabidopsis Ku80 deletion on the integration of the left border of T-DNA into plant chromosomal DNA via Agrobacterium tumefaciens | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yoshihara, Ryouhei
× Yoshihara, Ryouhei× Mitomi, Yuka× Okada, Maki× Shibata, Hanako× Tanokami, Mai× Nakajima, Yurie× Inui, Hideyuki× 大野, 豊× Furudate, Hiroyuki× Yutaka, Ono |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | T-DNA integration into plant chromosomal DNA via Agrobacterium tumefaciens can be achieved by exploiting the double-strand break repair system of the host’s DNA. However, the detailed mechanism of T-DNA integration remains unclear. Here, a sequence analysis of the junction sequences of T-DNA and chromosomal DNA was performed to assess the mechanism of T-DNA integration. T-DNA was introduced into Arabidopsis wild-type and NHEJ-deficient ku80-mutant plants using the floral dip method; the junctions of the left border (LB) of T-DNA were subsequently analyzed by adapter PCR. The most frequent junction of the LB of T-DNA to chromosomal DNA (LB-to-ChrDNA) was of the filler DNA type in both lines. The lengths of direct or inverted repeat sequences within or around the filler DNA sequence were longer in the ku80 mutant. In addition, the frequency of T-DNA integration near the transcription start site was significantly higher in the ku80 mutant. Our observations suggest that the presence of the Ku80 protein affects the location of the integration of T-DNA and the pattern of the formation of repeat sequences within or around the filler DNA during LB integration into chromosomal DNA. | |||||
| 書誌情報 |
Genes & Genetic Systems 巻 95, 号 4, p. 173-182, 発行日 2020-08 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1341-7568 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1266/ggs.19-00064 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.jstage.jst.go.jp/article/ggs/95/4/95_19-00064/_article/-char/ja | |||||
