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Live cell imaging of repair of EGFP plasmid DNA damage induced by X-ray energy deposition on hydrated waters
https://repo.qst.go.jp/records/80773
https://repo.qst.go.jp/records/80773979a5325-0157-42be-98db-83e8ee9537de
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-10-16 | |||||
| タイトル | ||||||
| タイトル | Live cell imaging of repair of EGFP plasmid DNA damage induced by X-ray energy deposition on hydrated waters | |||||
| タイトル | ||||||
| タイトル | 水和水に対するX線エネルギー付与により誘発されたEGFPプラスミドDNA損傷の修復のライブセルイメージング | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
平嵜, 敬志朗
× 平嵜, 敬志朗× 小畑, 結衣× 横谷, 明徳× Hirasaki, Keishiro× Obata, Yui× Yokoya, Akinari |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Studies using in vitro analysis reported that yields of the clustered DNA damage consisting base lesions, AP sites, or single-strand breaks increased with increasing level of hydration. Energy deposition pattern of ionizing irradiations to the DNA hydration network is obviously a key factor to determine the localization of lesions. So far, little evidence has supported the idea that the radiation induced cluster damage inhibits cellular repair activities. We developed an ex vivo procedure to analyze the reparability in cells using an EGFP-expressing plasmid. This method visualizes repairability of damage as an EGFP expression rate by transfection of the plasmids into non-irradiated mammalian cells. In the present study, we applied this method to investigate correlation between damage complexity and reparability in cells. The plasmids were exposed to X-rays in a Tris-EDTA buffer solution or fully hydrated films containing 35 water molecules per nucleotide. The absorbed dose gave the plasmid average one single-strand break according to Poisson statistics, i.e. the dose giving 37% (1/e) of a residual intact closed circular fraction of the plasmid. The irradiated plasmids were then transfected into non-irradiated human cells (MCF-7). EGFP expression in cells was observed for 48h with a fluorescence microscope. The repair rates were determined from the slops of the EGFP expression kinetics. Preliminary results showed that the efficiency for the hydrated plasmid films was lower than that for the solution sample. Hydration waters would contribute to complexity of DNA damage which might be less repairable rather than those by water radiolysis radicals. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 日本放射線影響学会第63回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2020-10-15 | |||||
| 日付タイプ | Issued | |||||
