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Effects of Reaction Environments on Radical-Scavenging Mechanisms of Ascorbic Acid
https://repo.qst.go.jp/records/80715
https://repo.qst.go.jp/records/8071534d4f391-71c5-4f43-bf5d-0c9f63461e0f
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-09-04 | |||||
| タイトル | ||||||
| タイトル | Effects of Reaction Environments on Radical-Scavenging Mechanisms of Ascorbic Acid | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakanishi, Ikuo
× Nakanishi, Ikuo× Shoji, Yoshimi× Ohkubo, Kei× Fukuhara, Kiyoshi× Ozawa, Toshihiko× Ken-ichiro, Matsumoto× Fukuzumi, Shunichi× Ikuo, Nakanishi× Yoshimi, Shoji× Kei, Ohkubo× Toshihiko, Ozawa× Kenichiro, Matsumoto |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The effect of reaction environment on the radical-scavenging mechanism of ascorbic acid (AscH2) was investigated using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) as a reactivity model of reactive oxygen species. Water-insoluble DPPH was solubilized by beta-cyclodextrin (beta-CD) in water to use DPPH in phosphate buffer solutions. The DPPH-scavenging rate of AscH2 in methanol (MeOH) was much slower than that in phosphate buffer (0.05 M, pH 7.0), where AscH2 undergoes deprotonation to produced ascorbate anion (AscH-). An organic soluble 5,6-isopropylidene-L-ascorbic acid (iAscH2) was also used to compare the DPPH-scavenging reactivity in MeOH and acetonitrile (MeCN). iAscH2 scavenged DPPH much slower in MeCN than in MeOH. In MeOH, Mg(ClO4)2 significantly decelerated the DPPH-scavenging reaction by AscH2 and iAscH2, while no effect of Mg(ClO4)2 on the rate of the reaction of iAscH2 with DPPH was observed in MeCN. On the other hand, Mg(ClO4)2 significantly accelerated the reaction between AscH2 and beta-CD-solubilized DPPH (DPPH/beta-CD) in phosphate buffer (0.05 M, pH 6.5), although the addition of 0.05 M Mg(ClO4)2 to the AscH2-DPPH/beta-CD system in phosphate buffer (0.05 M, pH 7.0) resulted in the change in pH of the phosphate buffer to be 6.5. Thus, DPPH-scavenging reaction by iAscH2 in MeCN may proceed via a one-step hydrogen-atom transfer as the rate-determining step, while an electron transfer pathway is partly involved in the reaction between AscH2 and DPPH/beta-CD in phosphate buffer solution, although the detailed mechanism for the deceleration effect of Mg(ClO4)2 in MeOH is under investigation. These results demonstrate that the DPPH-scavenging mechanism by AscH2 are affected by the reaction environments. | |||||
| 書誌情報 |
Journal of Clinical Biochemistry and Nutrition 巻 68, 号 2, p. 116-122, 発行日 2021-03 |
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| 出版者 | ||||||
| 出版者 | The Society for Free Radical Research Japan | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0912-0009 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3164/jcbn.20-147 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.jstage.jst.go.jp/article/jcbn/advpub/0/advpub_20-147/_article | |||||
