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SAXS Analysis of the Magnetorecepter Candidate Protein ISCA1
https://repo.qst.go.jp/records/80711
https://repo.qst.go.jp/records/80711ae969cdf-6e54-4a72-bced-0c466f139686
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-06-26 | |||||
| タイトル | ||||||
| タイトル | SAXS Analysis of the Magnetorecepter Candidate Protein ISCA1 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Arai, Shigeki
× Arai, Shigeki× Shimizu, Rumi× Adachi, Motoyasu× Ajito, Satoshi× Hirai, Mitsuhiro× Shigeki, Arai× Rumi, Shimizu× Motoyasu, Adachi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The quantum mechanism for magnetoreception in the biological system has been mainly investigated using cryptochromes (Crys) [Ritz T, et al., Biophys J. (2000) 78, 707; Maeda K, et al., PNAS. (2012) 109, 4774]. However, the signal transduction mechanism from Cry to the nervous system and the brain has not been elucidated yet. Recently, the ISCA1 (also called MagR) protein of pigeon, fruit fly, etc. was found to interact with Cry. The pigeon Cry4/ISCA1 (pCry4/pISCA1) complex orientates along the weak magnetic field (0.4 G) under blue light [Qin S, et al., Nat Mater. (2016) 15, 217], suggesting that ISCA1 is one of the candidates for partners of Cry upon the magnetoreception reaction and/or the signal transduction. In order to elucidate the structure and molecular behavior of pISCA1, we conducted the small angle X-ray scattering (SAXS) analysis of pISCA1. The result indicated that pISCA1 forms two types of conformers; the globular type-A and the rod-like type-B. The type-A conformer was self-polymerized larger than tetramer. Molecular modeling of the pISCA1 based on the SAXS analysis and the crystal structures of homologs (PDB: 2D2A, 1R94 and 1X0G) suggested that Fe-S clusters bind to the interfaces between the type-A pISCA1 molecules in its own oligomer. The conformational change from type-B to type-A and the subsequent self-polymerization of pISCA1 should enable to bind many Fe-S clusters that might improve the magnetomotive force of the pCry4/pISCA1 complex to orientate along the external magnetic field. This work was supported by JSPS KAKENHI Grant Number JP18K06174. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 2020WCPS | |||||
| 発表年月日 | ||||||
| 日付 | 2020-07-07 | |||||
| 日付タイプ | Issued | |||||
