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Targeted nuclear irradiation with an X-ray microbeam enhances total JC-1 fluorescence from mitochondria
https://repo.qst.go.jp/records/80689
https://repo.qst.go.jp/records/80689766eaf59-394f-42ea-a2c8-ff8c4db19121
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-10-11 | |||||
| タイトル | ||||||
| タイトル | Targeted nuclear irradiation with an X-ray microbeam enhances total JC-1 fluorescence from mitochondria | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kaminaga, Kiichi
× Kaminaga, Kiichi× Hamada, Ryo× Usami, Noriko× Suzuki, Keiji× Yokoya, Akinari× Kiichi, Kaminaga× Ryo, Hamada× Akinari, Yokoya |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Several studies have demonstrated that mitochondria are critically involved in the pleiotropic manifestation of radiation effects. While conventional whole-cell irradiation compromises the function of mitochondria, the effects of subcellular targeted irradiation are not yet fully understood. In this study, normal human diploid cells with cell-cycle indicators were exposed to a synchrotron X-ray microbeam, and mitochondrial membrane potential was quantified by JC-1 over the 72-h period after irradiation. Cytoplasmic irradiation was observed to temporarily enlarge the mitochondrial area with high membrane potential, while the total mitochondrial area did not change significantly. Unexpectedly, cell-nucleus irradiation promoted a similar increase not only in the mitochondrial areas with high membrane potential, but also in those with low membrane potential, which gave rise to the apparent increase in the total mitochondrial area. Augmentation of the mitochondrial area with low membrane potential was predominantly observed among G1 cells, suggesting that nucleus irradiation during the G1 phase regulated the mitochondrial dynamics of the cytoplasm, presumably through DNA damage in the nucleus | |||||
| 書誌情報 |
Radiation Research 巻 194, 号 5, p. 511-518, 発行日 2020-10 |
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| 出版者 | ||||||
| 出版者 | Radiation Research Society | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1667/RR15110.1 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1667/RR15110.1 | |||||
