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The effect of radiation on the ability of rat mammary cells to form mammospheres
https://repo.qst.go.jp/records/80390
https://repo.qst.go.jp/records/803902902c5a3-4bb3-449a-b97a-b9263debe90a
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-08-29 | |||||
| タイトル | ||||||
| タイトル | The effect of radiation on the ability of rat mammary cells to form mammospheres | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hosoki, Ayaka
× Hosoki, Ayaka× Ogawa, Mari× Nishimura, Yukiko× Nishimura, Mayumi× Daino, Kazuhiro× Kakinuma, Shizuko× Shimada, Yoshiya× Imaoka, Tatsuhiko× Ayaka, Hosoki× Mari, Ogawa× Yukiko, Nishimura× Mayumi, Nishimura× Kazuhiro, Daino× Shizuko, Kakinuma× Yoshiya, Shimada× Tatsuhiko, Imaoka |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | As classical transplantation repopulation assays for studying the radiobiology of rat mammary stem/progenitor cells are extremely time-consuming, this study aimed to characterize the radiobiological properties of mammospheres, spherical clumps of mammary cells formed under non-adherent culture conditions, which are a simple and widely used technique for assessing progenitor cell activity. Rat mammary cells were dissociated and used in transplantation repopulation assays and for the formation of mammospheres. Immunofluorescence for cytokeratin 14 and 18 was used to identify basal and luminal mammary epithelial cells, respectively. Incorporation of 5-bromo-2’-deoxyuridine (BrdU) was used to evaluate cell proliferation. The repopulating activity of the transplanted primary rat mammary cells demonstrated their radiosensitivity, reproducing previous data, with a significant reduction in repopulating activity at ≥2 Gy. Cells constituting rat mammospheres were positive for either cytokeratin 14 or 18, with occasional double-positive cells. Both proliferation and aggregation contributed to sphere formation. Cells obtained from the spheres showed lower repopulating activity after transplantation than primary cells. When primary cells were irradiated and then used for sphere formation, the efficiency of sphere formation was significantly decreased at 8 Gy but not at ≤6 Gy, indicating radioresistance of the formation process. Irradiation at 8 Gy reduced the proliferation of cells during sphere formation, whereas the cellular composition of the resulting spheres was unaffected. Thus, mammosphere formation assays may measure a property of putative mammary progenitors that is different from what is measured in the classic transplantation repopulation assay in radiobiology. | |||||
| 書誌情報 |
Radiation and Environmental Biophysics 巻 59, p. 711-721, 発行日 2020-08 |
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| 出版者 | ||||||
| 出版者 | Springer | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0301-634X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1007/s00411-020-00869-4 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://link.springer.com/article/10.1007/s00411-020-00869-4 | |||||
