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Involvement of SPRTN in DNA protein cross-link damage repair
https://repo.qst.go.jp/records/80335
https://repo.qst.go.jp/records/803356b910066-5640-4fbb-8b10-d97c7bef712f
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-08-18 | |||||
| タイトル | ||||||
| タイトル | Involvement of SPRTN in DNA protein cross-link damage repair | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakano, Toshiaki
× Nakano, Toshiaki× Sasanum, Hiroyuki× Tuda, Masataka× Hirota, Kouji× Shikazono, Naoya× Kawanishi, Masanobu× Takeda, Shunichi× Ide, Hiroshi× Tano, Keizo× Nakano, Toshiaki× Shikazono, Naoya |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | DNA-protein cross links (DPCs) are caused by covalently linking DNA and DNA-associated proteins and by intermediates of DNA-metabolizing enzymes. Current studies showed that SPRTN, metalloprotease, is a critical factor in proteolytic cleavage of DPCs. However, it has not been clarified which proteins are targets for SPRTN. In this study, we examined the contribution of SPRTN to cleave DPCs using a reverse genetic strategy with human lymphocytes, TK6. Cells deficient in SPRTN were not sensitive to formaldehyde (FA) producing DPCs with various proteins. In contrast, these cells showed hyper-sensitive to aza-deoxycytidine (azadC) which specifically trapped methyltransferase (Dnmt1) to DNA (Dnmt1-DPCs). AzadC treatment resulted in the accumulation of Dnmt1-DPCs in purified genomic DNA. The rate of removal of Dnmt1-DPCs was slower in sprtn-deficient cells than in wild type cells. Our data suggest that SPRTN plays critical role in the resolution of Dnmt1-DPCs in human cells. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第43回 日本分子生物学会 | |||||
| 発表年月日 | ||||||
| 日付 | 2020-12-02 | |||||
| 日付タイプ | Issued | |||||
