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PIN FORMED 2 Modulates the Transport of Arsenite in Arabidopsis thaliana
https://repo.qst.go.jp/records/80306
https://repo.qst.go.jp/records/80306fba202f3-a61d-4c18-9a76-c62211fe3b1e
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-08-07 | |||||
| タイトル | ||||||
| タイトル | PIN FORMED 2 Modulates the Transport of Arsenite in Arabidopsis thaliana | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Arif Ashraf, Mohammad
× Arif Ashraf, Mohammad× Umetsu, Kana× Ponomarenko, Olena× Saito, Michiko× Aslam, Mohammad× Antipova, Olga× Dolgova, Natalia× D Kiani, Cheyenne× Nehzati, Susan× Tanoi, Keitaro× Minegishi, Katsuyuki× Nagatsu, Kotaro× Kamiya, Takehiro× Fujiwara, Toru× Luschnig, Christian× Tanino, Karen× Pickering, Ingrid× N. George, Graham× Rahman, Abidur× Tanoi, Keitaro× Minegishi, Katsuyuki× Nagatsu, Kotaro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Arsenic contamination is a major environmental issue, as it may lead to serious health hazard. The reduced trivalent form of inorganic arsenic, arsenite, is in general more toxic to plants compared with the fully oxidized pentavalent arsenate. The uptake of arsenite in plants has been shown to be mediated through a large subfamily of plant aquaglyceroporins, nodulin 26-like intrinsic proteins (NIPs). However, the efflux mechanisms, as well as the mechanism of arsenite-induced root growth inhibition, remain poorly understood. Using molecular physiology, synchrotron imaging, and root transport assay approaches, we show that the cellular transport of trivalent arsenicals in Arabidopsis thaliana is strongly modulated by PIN FORMED 2 (PIN2) auxin efflux transporter. Root transport assay using radioactive arsenite, X-ray fluorescence imaging (XFI) coupled with X-ray absorption spectroscopy (XAS), and inductively coupled plasma mass spectrometry analysis revealed that pin2 plants accumulate higher concentrations of arsenite in roots compared with the wild-type. At the cellular level, arsenite specifically targets intracellular sorting of PIN2 and thereby alters the cellular auxin homeostasis. Consistently, loss of PIN2 function results in arsenite hypersensitivity in roots. XFI coupled with XAS further revealed that loss of PIN2 function results in specific accumulation of arsenical species, but not the other metals such as iron, zinc, or calcium in the root tip. Collectively, these results suggest that PIN2 likely functions as an arsenite efflux transporter for the distribution of arsenical species in planta. | |||||
| 書誌情報 |
Plant Communications 巻 1, 号 3, 発行日 2019-11 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2590-3462 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.xplc.2019.100009 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.sciencedirect.com/science/article/pii/S2590346219300094 | |||||
