WEKO3
アイテム
Cytoplasmic radiation induced radio-adaptive response in human lung fibroblast WI-38 cells
https://repo.qst.go.jp/records/80294
https://repo.qst.go.jp/records/8029480b388a0-4a3e-4463-8345-999aecc8791c
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-05-25 | |||||
| タイトル | ||||||
| タイトル | Cytoplasmic radiation induced radio-adaptive response in human lung fibroblast WI-38 cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Wang, Jun
× Wang, Jun× Kobayashi, Alisa× Ohsawa, Daisuke× Oikawa, Masakazu× Konishi, Teruaki× Kobayashi, Alisa× Ohsawa, Daisuke× Oikawa, Masakazu× Konishi, Teruaki |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | It has been reported that in low dose irradiated cells, radio-adaptive responses can be induced which make cells refractory to following high dose irradiation. However, whether adaptive responses can be induced in cells whose cytoplasm is hit while nucleus is not targeted remains unknown. In this study, by using the Single Particle Irradiation System to Cells proton microbeam facility at the National Institute of Radiological Sciences (Japan), we found that cytoplasmic irradiation activated the radio-adaptive responses in normal human lung fibroblast WI-38 cells. Our results showed that cytoplasmic irradiation with 500 protons prior to 2 Gy or 6 Gy X-ray broad beam irradiation resulted in obvious decrease of the DNA double-strands breaks levels. Further, the radio-adaptive responses were not induced in cells whose cytoplasm was hit with not higher than 100 protons. And a longer than 6 hours’ time interval between cytoplasmic irradiation and high dose X-ray broad beam irradiation was needed for the induction of adaptive responses. In addition, cytoplasmic irradiation elevated mitochondrial superoxide level, which further enhanced the phosphorylation of ERK 1/2 (Extracellular signal-regulated kinases 1/2) and its mediated nucleus accumulation of NRF2 (nuclear factor (erythroid-derived 2)-like 2). This signaling way contributed to the induction of the radio-adaptive response in cytoplasm irradiated cells as manifested by using the selective scavenger or inhibitors of mitochondrial superoxide, ERK 1/2 and NRF2. Overall, we confirmed that cytoplasmic irradiation was capable to induce radio-adaptive responses, and demonstrated the activated mitochondrial superoxide/ ERK 1/2/ NRF2 signaling was one of the underlying mechanisms. Our results brought interesting information to cytoplasm targeted irradiation induced biological effects. | |||||
| 書誌情報 |
Radiation Research 巻 194, p. 288-297, 発行日 2020-07 |
|||||
| 出版者 | ||||||
| 出版者 | Radiation Research Society | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1667/RR15575.1 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://meridian.allenpress.com/radiation-research/article/doi/10.1667/RR15575.1/441606/Cytoplasmic-Radiation-Induced-Radio-Adaptive | |||||
