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Characterization of a Novel Murine Colon Carcinoma Subline with High-Metastatic Activity Established by in Vivo Selection Method
https://repo.qst.go.jp/records/79989
https://repo.qst.go.jp/records/79989f4f559d0-9204-4c35-aa7b-1e3a62a79949
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-05-29 | |||||
| タイトル | ||||||
| タイトル | Characterization of a Novel Murine Colon Carcinoma Subline with High-Metastatic Activity Established by in Vivo Selection Method | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ma, Liqiu
× Ma, Liqiu× Sakamoto, Yoshimitsu× Kanai, Akinori× Ohtsuka, Hiromi× Takahashi, Akihisa× Kakimi, Kazuhiro× Imai, Takashi× Shimokawa, Takashi× Ma, Liqiu× Yoshimitsu, Sakamoto× Hiromi, Ohtsuka× Akihisa, Takahashi× Takashi, Imai× Takashi, Shimokawa |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The establishment of cancer cell lines, which have different metastatic abilities compared with the parental cell, is considered as an effective approach to investigate mechanisms of metastasis. A highly metastatic potential mouse colon cancer cell subline, Colon-26MGS, was derived from the parental cell line Colon-26 by in vivo selection using continuous subcutaneous implanting to immunocompetent mice. To clarify the mechanisms involved in the enhancement of metastasis, morphological characteristics, cell proliferation, and gene expression profiles were compared between Colon-26MGS and the parental cell. Colon-26MGS showed over 10 times higher metastatic ability compared with the parental cell, but there were no differences in morphological characteristics and in vitro proliferation rates. In addition, the Colon-26MGS-bearing mice exhibited no marked change of splenocyte population and lung pre-metastatic niche with tumor-free mice, but there were significant differences compared to Colon-26-bearing mice. RNA-seq analyses indicated that immune costimulatory molecules were significantly up-regulated in Colon-26MGS. These results suggest that Colon-26MGS showed not only higher metastatic activity, but also less induction property of host immune response compared to parental Colon-26. Colon-26MGS has proven to be a novel useful tool for studying multiple mechanisms involving metastasis enhancement. | |||||
| 書誌情報 |
International Journal of Molecular Sciences 巻 21, 号 8, p. 2829, 発行日 2020-04 |
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| 出版者 | ||||||
| 出版者 | MDPI | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1422-0067 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 32325684 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3390/ijms21082829 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.mdpi.com/1422-0067/21/8/2829 | |||||
