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Effect of Ion Beams and Gamma Rays Irradiation on Mutation Induction in Bacillus subtilis Spores
https://repo.qst.go.jp/records/79305
https://repo.qst.go.jp/records/79305a73e10af-4c6e-4a9b-a828-48045649260a
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-03-11 | |||||
| タイトル | ||||||
| タイトル | Effect of Ion Beams and Gamma Rays Irradiation on Mutation Induction in Bacillus subtilis Spores | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Huynh Phuong Uyen , Nguyen
× Huynh Phuong Uyen , Nguyen× Sakai, Toshio× Den, Hiroki× Furuta, Masahiro× Sato, Katsuya× Ono, Yutaka× Sato, Katsuya× Ono, Yutaka |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Bacillus subtilis is known as Gram-positive, rod-shaped, aerobic, spore-forming bacterium that is naturally found in soil. The spore is resistant to environmental factors such as heat, desiccation, chemical, and radiation. Besides, ionizing radiation induces DNA damages including single-strand breaks and double-strand break and generates reactive oxygen species which also induces DNA strand breaks. Ion beams have a high LET and give DNA damage containing double-strand break locally (clustered damage) than gamma rays do. Recently, ion beams have been used for mutation breeding in biological objects including rice, flowers and bacteria. Mutations in the RNA polymerase β subunit of the B. subtilis, encoded by rpoB gene, related to rifampicin resistance and can be genareted by irradiation method. In this work, we utilized ion beams to check mutation induction for rifampicin to characterize the mutation by high LET ion beam irradiation to B. subtilis spores. The initial spore germination process seemed not to be affected by ion–beam irradiation. Outgrowth phase after the germination became longer with the irradiation dose as well as 60Co gamma-rays. These results suggest that damaged sites in spores are similar between gamma- and ion-beam irradiations. By the screening of RifR mutants, a certain number of RifR mutants was obtained for 4He2+, 60Co, 40Ar13+ irradiation within lower doses showing relatively lower lethality. | |||||
| 書誌情報 |
QST Takasaki Annual Report 2018 巻 QST-M-23, p. 84, 発行日 2020-03 |
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