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Characteristics of induced mutations in offspring derived from irradiated mouse spermatogonia and mature oocytes.
https://repo.qst.go.jp/records/79227
https://repo.qst.go.jp/records/7922780df8d22-e370-4f28-84ac-aa2a2e922442
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-03-06 | |||||
| タイトル | ||||||
| タイトル | Characteristics of induced mutations in offspring derived from irradiated mouse spermatogonia and mature oocytes. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Satoh, Yasunari
× Satoh, Yasunari× Jun-Ichi, Asakawa× Nishimura, Mayumi× Kuo, Tony× Shinkai, Norio× M Cullings, Harry× Minakuchi, Yohei× Sese, Jun× Toyoda, Atsushi× Shimada, Yoshiya× Nakamura, Nori× Uchimura, Arikuni× Asakawa, Jun-ichi× Mayumi, Nishimura× Yoshiya, Shimada |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The exposure of germ cells to radiation introduces mutations in the genomes of offspring, and a previous whole-genome sequencing study indicated that the irradiation of mouse sperm induces insertions/deletions (indels) and multisite mutations (clustered single nucleotide variants and indels). However, the current knowledge on the mutation spectra is limited, and the effects of radiation exposure on germ cells at stages other than the sperm stage remain unknown. Here, we performed whole-genome sequencing experiments to investigate the exposure of spermatogonia and mature oocytes. We compared de novo mutations in a total of 24 F1 mice conceived before and after the irradiation of their parents. The results indicated that radiation exposure, 4 Gy of gamma rays, induced 9.6 indels and 2.5 multisite mutations in spermatogonia and 4.7 indels and 3.1 multisite mutations in mature oocytes in the autosomal regions of each F1 individual. Notably, we found two types of deletions, namely, small deletions (mainly 1~12 nucleotides) in non-repeat sequences, many of which showed microhomology at the breakpoint junction, and single-nucleotide deletions in mononucleotide repeat sequences. The results suggest that these deletions and multisite mutations could be a typical signature of mutations induced by parental irradiation in mammals. | |||||
| 書誌情報 |
Scientific Reports 巻 10, 号 1, p. 37, 発行日 2020-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2045-2322 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 31913321 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1038/s41598-019-56881-2 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.nature.com/articles/s41598-019-56881-2 | |||||
