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Potential mechanisms for Protective Effect of D-Methionine on Plasmid DNA Damage Induced by Therapeutic Carbon Ions
https://repo.qst.go.jp/records/79152
https://repo.qst.go.jp/records/79152534bc4d4-6624-4ecb-99b1-8aa928eda775
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-12-12 | |||||
| タイトル | ||||||
| タイトル | Potential mechanisms for Protective Effect of D-Methionine on Plasmid DNA Damage Induced by Therapeutic Carbon Ions | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yogo, Katsunori
× Yogo, Katsunori× Murayama, Chieko× Fujisawa, Yoshiki× Maeyama, Takuya× Hirayama, Ryoichi× Ogawa, Yukihiro× Ken-ichiro, Matsumoto× Nakanishi, Ikuo× Yasuda, Hiroshi× Ishiyama, Hiromichi× Hayakawa, Kazushige× Yogo, Katsunori× Murayama, Chieko× Fujisawa, Yoshiki× Maeyama, Takuya× Hirayama, Ryoichi× Ogawa, Yukihiro× Matsumoto, Kenichiro× Nakanishi, Ikuo× Yasuda, Hiroshi× Hayakawa, Kazushige |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | D-methionine (D-Met), a dextrorotatory isoform of the amino acid L-methionine (L-Met), can prevent oral mucositis and salivary hypofunction in mice exposed to radiation. However, the mechanism of its radioprotection is unclear, especially with regard to the stereospecific functions of D-Met. Radiation is known to cause injury to normal tissue by triggering DNA damage in cells. Thus, this study investigated whether the chirality of D-/L-Met affects radiation-induced events at the DNA level. We selected plasmid DNA assays to examine this effect in vitro, as these assays are highly sensitive and allow easy detection of DNA damage. Samples of supercoiled pBR322 plasmid DNA mixed with D-Met, L-Met, or dimethylsulfoxide (DMSO) were prepared and irradiated with a Bragg peak beam of carbon ions (~290 MeV/u) with a 6 cm spread. DNA strand breaks were detected by the change in the form of the plasmid and were subsequently quantified by agarose gel electrophoresis. We found that D-Met yielded approximately equivalent protection from carbon-ion-induced DNA damage as DMSO. Thus, we propose that the protective functions of Met against plasmid DNA damage could be explained by the same mechanism as that for DMSO, namely, hydroxyl radical scavenging. This stereospecific radioprotective mechanism occurred at a level other than DNA level. There was no significant difference between the radioprotective effect of D-Met and L-Met on DNA. | |||||
| 書誌情報 |
Radiation Research 巻 193, 号 6, p. 513-519, 発行日 2020-03 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1667/RR15502.1 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.rrjournal.org/doi/abs/10.1667/RR15502.1?= | |||||
