WEKO3
アイテム
Positive torsional stress on DNA enhances unwrapping of nucleosomal DNA
https://repo.qst.go.jp/records/79043
https://repo.qst.go.jp/records/790434d4b346e-c268-4177-a729-107077f7367f
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-02-17 | |||||
| タイトル | ||||||
| タイトル | Positive torsional stress on DNA enhances unwrapping of nucleosomal DNA | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ishida, Hisashi
× Ishida, Hisashi× Kono, Hidetoshi× Ishida, Hisashi× Kono, Hidetoshi |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Torsional stress has a significant impact on the structure and stability of the nucleosome. RNA polymerase imposes torsional stress on the DNA in chromatin and unwraps the DNA from the nucleosome to access the genetic information encoded in the DNA. Some chromatin Remodelers are thought to change nucleosome positioning by adding torsional stress on DNA. To understand how such torsional stress affects the stability of the nucleosome, we examined the unwraping of the outer superhelical turn of the nucleosomal DNA under positive or negative torsional stress with all-atom molecular dynamics simulations. The free energies for unwrapping the DNA indicate that positive stress that overtwists DNA facilitates asymmetric unwrapping of the DNA while negative stress that undertwists DNA does not. Under positive stress, DNA was straightened and rigid, which facilitated a large-scale asymmetric unwrapping of the DNA without a large extension of two DNA.In the structure, one end of the DNA was disconnected from H3 and H2A/H2B while the other end of the DNA stably remained wrapped. Under negative stress, the DNA became more bendable and flexible, which facilitated the binding of the unwrapped DNA to the octamer in a stable state. Our results suggest that the stability of individual nucleosomes in the chromatin can be changed by torsional stress, thereby DNA accessibility of regulatory proteins can be produced to maintain gene expression without large conformational changes even in heterochromatin. |
|||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Biophysical Society Annual Meeting 2020 | |||||
| 発表年月日 | ||||||
| 日付 | 2020-02-18 | |||||
| 日付タイプ | Issued | |||||
