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The Importance of Primary and Secondary Bystander Effects Cross-Talk between Human Lung Cancer and Lung Normal Cells after Proton Microbeam Irradiation (S16-IOL02/FY2018)
https://repo.qst.go.jp/records/78557
https://repo.qst.go.jp/records/78557bc6ba6fe-8b3e-4785-8deb-5d424efc30ed
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-03-22 | |||||
| タイトル | ||||||
| タイトル | The Importance of Primary and Secondary Bystander Effects Cross-Talk between Human Lung Cancer and Lung Normal Cells after Proton Microbeam Irradiation (S16-IOL02/FY2018) | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Autsavapormporn, Narongchai
× Autsavapormporn, Narongchai× Liu, Cuihua× Ahbrizal Tengku Ahmad, Tengku× Kobayashi, Alisa× Oikawa, Masakazu× Furusawa, Yoshiya× Ohsawa, Daisuke× Konishi, Teruaki× Liu, Cuihua× Kobayashi, Alisa× Oikawa, Masakazu× Furusawa, Yoshiya× Ohsawa, Daisuke× Konishi, Teruaki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | 1.Purpose and specific aim: The objective of this study is to investigate the role and mechanism underlying of GJIC in determining human response to proton radiation. This study particularly focused on the communication of the primary bystander signaling events between protons-irradiated cancer cells/normal cells and bystander normal cells. In addition, the secondary bystander signaling events between the primary bystander normal cells and the secondary bystander normal cells will also be determined. Communication of stressful or protective effect between human lung cancer cells and human lung normal cells after protons microbeam irradiation may amplify or mitigate the damage in bystander normal cells. The radiation studies outlined in the project have been initiated during Run at the SPICE-BIO, NIRS-QST during FY2016-2018. In studies related to the 2 specific aims to examine the following: 1. Test the hypothesis that the primary-and secondary bystander responses in normal cells occur at particle fluences so low that only 0.036-0.4% of cancer cells in a culture dish are traversed by protons. 2. To examine the protective effect of gap-junction inhibitor (AGA) and carboxy-PTIO (NO inhibitor) in the secondary bystander normal cells. 3. To investigate effects of bystander response on cancer cells and normal cells after proton microbeam irradiation under hypoxic conditions. 2. Abstract Increased understanding of radiation-induced secondary bystander effect (RISBE) is relevant to radiation therapy since it likely contributes to normal tissue injury and tumor recurrence, subsequently resulting in treatment failure. In this work, we developed a simple method based on proton microbeam radiation and a transwell insert co-culture system to elucidate the RISBE between irradiation human lung cancer cells and nonirradiated human normal cells. A549 lung cancer cells received a single dose or fractionated doses of proton microbeam radiation to generate the primary bystander cells. These cells were then seeded on the top of the insert with secondary bystander WI-38 normal cells growing underneath in the presence or absence of gap junction intercellular communication (GJIC) inhibitor, 18-alpha-glycyrrhetnic acid (AGA). Cells were co-cultured before harvesting and assayed for micronucleus formation. The results of this work showed that fractionated doses of protons caused less DNA damage in the secondary bystander WI-38 cells compared to a single radiation dose, where the means differ by 20%. However, the damaging effect in the secondary bystander normal cells could be eliminated when treated with AGA. This novel work reflects our effort to demonstrate that GJIC play a major role in the RISBE generated from the primary bystander cancer cells. These projects were particularly successful since some part of these work were accepted to be published in Radiation Research, titled “Emerging role of secondary bystander effects induced by fractionated proton microbeam radiation.” Acknowledgements NA would like to thanks the Japanese Society for the Promoting of Science and the JSPS Alumni Association of Thailand (JATA) for the JSPS Bridge fellowship 2016 (BR161201). Authors would like to thank the NIRS-IOL program for the support and have accelerated our international collaboration. This study was supported in part by a JSPS KAKENHI Grant-in-Aid for Scientific Research B (17H04268). |
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| 書誌情報 |
平成３０年度 共用施設（PASTA＆SPICE、NASBEE、X/γ線照射装置）成果報告書 p. 29-31, 発行日 2019-12 |
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| 出版者 | ||||||
| 出版者 | 放射線医学総合研究所 加速器工学部 静電加速器運転室 | |||||
