WEKO3
アイテム
Cytogenetic Examination of Human Peripheral Blood Lymphocytes Cryopreserved after Gamma Irradiation: A Pilot Study
https://repo.qst.go.jp/records/78329
https://repo.qst.go.jp/records/7832920a4e78d-0212-49f6-869f-e5fb9292ed95
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-01-06 | |||||
| タイトル | ||||||
| タイトル | Cytogenetic Examination of Human Peripheral Blood Lymphocytes Cryopreserved after Gamma Irradiation: A Pilot Study | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Suto, Yumiko
× Suto, Yumiko× Tominaga, Takako× Akiyama, Miho× Hirai, Momoki× Suto, Yumiko× Tominaga, Takako× Akiyama, Miho× Hirai, Momoki |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | For the assessment of radiation-induced damage in human cells, the frequency of chromosomal aberrations, especially that of dicentric chromosomes in peripheral blood lymphocytes, has been used as the measure in biodosimetry. Considering mass-casualty radiological incidents, it is hoped to preserve part of the blood specimens to be examined at an appropriate time after the medical triage process. In this study, the potential usefulness of lymphocytes cryopreserved for longer than one year was assessed as a pilot study. Seven blood specimens from three healthy donors were experimentally irradiated and cryopreserved for approximately one to five years. All cryopreserved specimens examined were successfully cultured to obtain a sufficient number of mitotic cells for dosimetric analyses. Blood specimens from one donor were intensively examined for the precise comparative analysis of chromosomal aberrations between cultures of cryopreserved specimens and those of freshly prepared specimens. The frequencies of cells with chromosomal aberrations in 1.5-year-cryopreserved specimens after 2-Gy gamma irradiation were not significantly different from those examined using a fresh blood specimen. In this study, we showed the practical significance of the cryopreservation method of peripheral blood lymphocytes that can offer a new strategy of preparedness for triage in mass-casualty radiation incidents/accidents. | |||||
| 書誌情報 |
Cytologia 巻 85, 号 1, p. 71-77, 発行日 2020-03 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0011-4545 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1508/cytologia.85.71 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.jstage.jst.go.jp/article/cytologia/85/1/85_D-19-00101/_article/-char/ja/ | |||||
