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Repair susceptibility of X-irradiated EGFP plasmid DNA transfected into non-irradiated cells
https://repo.qst.go.jp/records/77947
https://repo.qst.go.jp/records/7794743e55dea-7e12-47c4-98ea-ca751b64d027
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-11-28 | |||||
| タイトル | ||||||
| タイトル | Repair susceptibility of X-irradiated EGFP plasmid DNA transfected into non-irradiated cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Obata, Yui
× Obata, Yui× Hirasaki, Keishiro× Yokoya, Akinari× Obata, Yui× Hirasaki, Keishiro× Yokoya, Akinari |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Ionizing radiation is known to cause various chemical damage to cells. Whole-cell irradiation inevitably causes damage not only in genomic DNA but also intracellular organelles. To investigate the repairability of DNA damage separately from the effects on organelles, EGFP-expressing plasmids were exposed to X-rays in solution and then transfected into non-irradiated human MCF-7 breast cancer cells, which induce a low expression of DNA damage response protein BRCA1. Live-cell imaging of EGFP fluorescence of the cells was performed to measure repair efficiency of the plasmids in the cells. The kinetics of the fluorescent expression after irradiation of several doses were compared with those treated with a nicking or restriction enzyme used as positive controls to induce single- (SSB) or double-strand breaks (DSB), respectively. Assuming a Poisson distribution of the strand breaks in the plasmid, expected kinetic curves were also calculated. The numbers of EGFP-expressing cells observed were considerably fewer than the calculated values. That is, the difficulty of DNA repair is peculiar to irradiation. These results suggest that the lower EGFP expression efficiencies were not only due to complex chemical structures of DNA-strand-break termini compared with those created by enzyme treatments, but also that localization of non-DSB type lesions might be facilitated by irradiation and thus compromise DNA repair efficiency. In the future, we test MCF-10A (BRCA1 positive) to reveal the role of DNA damage responses in the repair dynamics. We also conduct high-LET irradiation to cells to produce more dense ionizations in the plasmid. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第3回QST国際シンポジウム「Quantum Life Science」 | |||||
| 発表年月日 | ||||||
| 日付 | 2019-12-04 | |||||
| 日付タイプ | Issued | |||||
