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Changes of mitochondrial dynamics and ATP production after colchicine treatment or X-irradiation
https://repo.qst.go.jp/records/77889
https://repo.qst.go.jp/records/77889fab821bd-6d2d-42d7-820a-b56187b4545d
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-11-22 | |||||
| タイトル | ||||||
| タイトル | Changes of mitochondrial dynamics and ATP production after colchicine treatment or X-irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kimura, Yuka
× Kimura, Yuka× Ochi, Noriyuki× Yokoya, Akinari× Kimura, Yuka× Ochi, Noriyuki× Yokoya, Akinari |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This study aimed to reveal the effect of external stresses on mitochondrial dynamics. Mitochondria are involved in a crucial role to retain a homeostasis, such as ATP production, Ca2+ storage, and induction of apoptosis. In addition, it has been well known that mitochondria move very actively in cytoplasm, like bacteria. Although these motions may be involved in physiological function, the mechanistic process of this has not yet been understood. We predict that the mitochondrial motion is driven by polymerization and depolymerization of fibers of cytoskeletons. Therefore, we analyzed effects on the motions, as well as ATP production, caused by a tubulin polymerization inhibitor or X-irradiation damage. Mitochondria of human normal fibroblast cells were specifically stained by JC-1 which is a fluorescence dye visualizing mitochondrial sites of high and low membrane potential with red and green, respectively. As a maker of the motion, mitochondrial active sites were tracked by a time-lapse observation. Obtained results indicates that the velocity of the active site motion fitted well to a Levy distribution rather than Gaussian distribution, indicating that the normal mitochondria motion is not a random movement, but shows occasional long-distance steps. The frequency of the low velocity components was significantly increased by treatments with both colchicine treatment and X-irradiation. ATP production in cells was also quantified by the luciferin-luciferase method. The ATP concentration was upregulated by both treatments. These evidences indicate that X-rays would compromise the mitochondrial motion through damaging microtubules, and induce excess ATP molecules, which are not consumed for tubulin polymerization. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第3回QST国際シンポジウム「Quantum Life Science」 | |||||
| 発表年月日 | ||||||
| 日付 | 2019-12-04 | |||||
| 日付タイプ | Issued | |||||
