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Mitochondrial Dynamics in Mammalian Cells Modified by Colchicine Administration or X-irradiation
https://repo.qst.go.jp/records/77710
https://repo.qst.go.jp/records/7771001f30d7b-e9ae-4901-929b-5051a565070e
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-11-12 | |||||
| タイトル | ||||||
| タイトル | Mitochondrial Dynamics in Mammalian Cells Modified by Colchicine Administration or X-irradiation | |||||
| タイトル | ||||||
| タイトル | ルヒチン投与またはX線照射によって阻害される哺乳動物細胞におけるミトコンドリア動態 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
木村, 由佳
× 木村, 由佳× 大内, 則幸× 横谷, 明徳× Kimura, Yuka× Ochi, Noriyuki× Yokoya, Akinari |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The aim of this study is to reveal the effect of external stresses on mitochondrial dynamics. Mitochondria are involved in a crucial role to retain a homeostasis, such as ATP production, induction of apoptosis. In addition, it has been well known that mitochondria move very actively in cytoplasm, like bacteria, but the contribution of their rapid position change to physiological function has been less well understood. We analyzed the change of the bacterial motions of mitochondria caused by a tubulin polymerization inhibitor or X-irradiation. Mitochondria of human normal fibroblast cells were specifically stained by JC-1 which is a fluorescence dye visualizing mitochondrial sites of high and low membrane potential with red and green, respectively. Mitochondrial active sites were tracked by a time-lapse observation. The velocity of the active site motion fitted well to a Levy distribution rather than Gaussian distribution, indicating that the normal mitochondria motion is not a random movement, but shows occasional long-distance steps. The frequency of the low velocity components was significantly increased by treatments with both colchicine treatment and X-irradiation. ATP production in cells was also quantified by the luciferin-luciferase method. The ATP concentration was upregulated by both treatments. These evidences indicate that X-rays would compromise the mitochondrial motion through damaging microtubules, and induce excess ATP molecules, which are not consumed for tubulin polymerization. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 日本放射線影響学会第62回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2019-11-14 | |||||
| 日付タイプ | Issued | |||||
