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Presence of X-ray induced high concentrated hydrogen peroxide clusters
https://repo.qst.go.jp/records/77615
https://repo.qst.go.jp/records/77615cf7a4add-7d90-4e32-9d93-3b4c5e00ffbc
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-09-05 | |||||
| タイトル | ||||||
| タイトル | Presence of X-ray induced high concentrated hydrogen peroxide clusters | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ueno, Megumi
× Ueno, Megumi× Nakanishi, Ikuo× Ken-ichiro, Matsumoto× Ueno, Megumi× Nakanishi, Ikuo× Matsumoto, Kenichiro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Presence of high concetntrated hydrogen peroxide (H2O2) clusters in an aqueous solution during X-ray irradiation was demonstrated. A method to measure the distance between the clusters was attempted. X-ray induced H2O2 generation in the sample solution is related to the hydrogen-donor-independent reduction of nitroxyl radical, however the reaction mechanisms are still in progress. In this study, the relation of H2O2 concentraion and hydrogen-donor-independent reduction of nitroxyl radical was tested, and then the distance between localyzed H2O2 clusters was estimated. Reaction mixtures containing 0.1 mM of a nitroxyl redox probe, TEMPOL or Carbamoyl-PROXYL, were prepared with containing several different concentrations (1.0 mM‒9.8 M) of H2O2. The reaction mixture was irradiated by 12000 μW/cm2 UVB for 2 min, and the reduction of the EPR signal of the nitorxyl radical was measured. An aliquot (2 µL) of 200 mM K3[Fe(CN)6] solution was added to 200 µL of the reaction mixture, and the time course of the EPR signal of the nitorxyl radical was measured. A series of nitroxyl radical solutions with several different concentrations (0.06 μM‒2.28 mM) was irradiated with X-ray, and then the amount of reduction of nitroxyl radical in each sample was measured by EPR. The UV induced EPR signal loss of the nitorxyl radical was increased with H2O2 higher than 10 mM. The EPR signal of nitorxyl radicals were decreased in the solution containing 1 M of H2O2, when K3[Fe(CN)6] was added to the reaction mixture. Oxidative stimulation by UVB irradiation or addition of K3[Fe(CN)6] could cause nitorxyl radical reduction in a high concentration H2O2 environment. This result suggests that ionizing radiations could prepare a high concentration H2O2 environment, which may be localized. Plotting reduction amount with density of nitroxyl radical showed that the high concentration H2O2 clusters are with 43‒44 nm separation. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The Society for Redox Biology and Medicine's 26th Annual Conference (SfRBM 2019) | |||||
| 発表年月日 | ||||||
| 日付 | 2019-11-21 | |||||
| 日付タイプ | Issued | |||||
