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FISH analysis of chromosomal aberrations in splenocytes of Trp53-heterozygous mice exposed to both chronic restraint-induced stress and Fe-particle irradiation
https://repo.qst.go.jp/records/77559
https://repo.qst.go.jp/records/77559b7dc1a1f-e440-4c18-81c3-f471ac4eafc7
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-11-21 | |||||
| タイトル | ||||||
| タイトル | FISH analysis of chromosomal aberrations in splenocytes of Trp53-heterozygous mice exposed to both chronic restraint-induced stress and Fe-particle irradiation | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
勝部, 孝則
× 勝部, 孝則× 王, 冰× 田中, 薫× 二宮, 康晴× 平川, 博一× 劉, 翠華× 丸山, 耕一× Vares, Guillaume× LIU, Qiang× 中島, 徹夫× 藤森, 亮× 根井, 充× Katsube, Takanori× Wang, Bing× Tanaka, Kaoru× Ninomiya, Yasuharu× Hirakawa, Hirokazu× Liu, Cuihua× Maruyama, Kouichi× Vares, Guillaume× Nakajima, Tetsuo× Fujimori, Akira× Nenoi, Mitsuru |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Astronauts develop psychological stress (PS) during space flight because of enclosed environment, zero gravity and altered light-dark cycles. At the same time, they are exposed to cosmic rays including high atomic number and energy (HZE) particles such as iron-56 (Fe) ions. A pioneering study showed that chronic restraint-induced PS (CRIPS) could attenuate Trp53 functions and increase carcinogenesis induced by low linear energy transfer (LET) gamma-rays in Trp53-heterozygous (Trp53+/-) mice. To elucidate possible modification effects from CRIPS on high LET HZE particles-induced health consequences, Trp53+/- C57BL/6N male mice (six-week-old) were restrained 6 hours per day for consecutive 28 days, and total-body irradiation (TBI) of Fe ions at a dose of 0.1 or 2 Gy was given on the 8th day. Chromosomal aberrations (CAs) in splenocytes were observed by fluorescence in situ hybridization (FISH) at the end of the 28-day restraint regimen. Induction of PS in our experimental model was confirmed by an increase in urinary corticosterone level. CRIPS reduced splenocyte number per spleen but Fe TBI. Fe TBI alone increased CAs significantly at 2.0 Gy but 0.1 Gy. Exposure to CRIPS alone didn’t induce CAs but concurrent exposure to CRIPS and Fe TBI showed a tendency of increase in the frequency of CAs as compared to exposure to Fe TBI alone. Further analyses are required to confirm the modification effects from CRIPS on Fe TBI-induced CAs. This work was partially supported by the MEXT Grant-in-Aid for | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 日本放射線影響学会第62回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2019-11-14 | |||||
| 日付タイプ | Issued | |||||
