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Anti-tumor effects of [At-211]-MABG treatment in PC12 pheochromocytoma cells and cell death via p53-p21 signaling pathway
https://repo.qst.go.jp/records/77532
https://repo.qst.go.jp/records/77532f822f942-bfba-4294-8a77-8f09b4d7feea
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-09-10 | |||||
| タイトル | ||||||
| タイトル | Anti-tumor effects of [At-211]-MABG treatment in PC12 pheochromocytoma cells and cell death via p53-p21 signaling pathway | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
坂下, 哲哉
× 坂下, 哲哉× 大島, 康宏× 河野, 暢明× 横田, 裕一郎× 渡辺, 茂樹× 佐々木, 一郎× 石岡, 典子× 荒川, 和晴× Sakashita, Tetsuya× Oshima, Yasuhiro× Yokota, Yuuichiro× Watanabe, Shigeki× Sasaki, Ichiro× Ishioka, Noriko |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Targeted α-particle therapy is a promising option for patients with malignant pheochromocytoma. Recent observations of α-emitting meta-[At-211]-astato-benzylguanidine ([At-211]-MABG) in a pheochromocytoma mouse model showed a strong anti-tumor effect [1], though the molecular mechanism remained elusive. Here, we show the comprehensive RNA-sequencing (RNA-seq) data for rat pheochromocytoma cell line PC12 cells based on in vitro [At-211]-MABG administration experiments. Enrichment analysis of differentially expressed genes (DEGs) and analysis of the gene expression profiles of cell cycle checkpoints revealed the mode of cell death via the p53-p21 signaling pathway after [At-211]-MABG treatment. Only p53-targeted genes were ranked among representative DEGs for decreased survival at all time points. Also, we estimated the low fluence rate of α-particles in in vitro [At-211]-MABG administration, approximately “one α-particle emission per one cell in one hour” for 10% survival. Long-lasting p53-induced signaling may break the wall of the “low fluence rate” in PC12 tumor cell death to exert anti-tumor effects. We recently published these findings on RNA-seq of in vitro PC12 transcriptome, suggesting that p53-p21 signaling pathway was an important cell death mechanism in anti-tumor effect of [At-211]-MABG [2]. References: [1] Y. Ohshima et al., Eur J Nucl Med Mol Imaging, 2018. [2] Y. Ohshima et al., Theranostics, 2019 |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 日本放射線影響学会第62回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2019-11-15 | |||||
| 日付タイプ | Issued | |||||
