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G1 Premature Chromosome Condensation (PCC) Assay
https://repo.qst.go.jp/records/77522
https://repo.qst.go.jp/records/775220e485e41-ef4d-45ae-ad28-56172ba57b28
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-11-20 | |||||
| タイトル | ||||||
| タイトル | G1 Premature Chromosome Condensation (PCC) Assay | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Okayasu, Ryuichi
× Okayasu, Ryuichi× Liu, Cuihua× Okayasu, Ryuichi× Liu, Cuihua |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Premature chromosome condensation (PCC) is a sensitive and unique way to detect interphase chromosome damage and its recovery in mammalian cells irradiated with ionizing radiation. In this chapter, we describe G1 PCC assay with which one can measure immediate chromosome breaks in G1 type chromosomes and their repair/rejoining. In order to induce G1 PCC, one needs to fuse mitotic cells with G1 cells to be tested. There are two methods to fuse cells; one is to use Sendai virus or its equivalent, and another method needs polyethylene glycol (PEG) as a fusing agent. The date obtained with PCC assay can bridge the gap between radiation-induced DNA damage (mainly double strand breaks) and chromosome aberrations observable at metaphase stage. | |||||
| 書誌情報 |
G1 Premature Chromosome Condensation (PCC) Assay 発行日 2019-07 |
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| ISBN | ||||||
| 識別子タイプ | ISBN | |||||
| 関連識別子 | 978-1-4939-9430-4 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://link.springer.com/protocol/10.1007/978-1-4939-9432-8_4 | |||||
