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Radiation induced generation of localized highly concentrated hydrogen peroxide in water

https://repo.qst.go.jp/records/77464
https://repo.qst.go.jp/records/77464
36a4e33a-5b1e-406d-b443-4a8e2a4fc02f
Item type 会議発表用資料 / Presentation(1)
公開日 2019-07-22
タイトル
タイトル Radiation induced generation of localized highly concentrated hydrogen peroxide in water
言語
言語 jpn
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_c94f
資源タイプ conference object
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 上野, 恵美

× 上野, 恵美

WEKO 802878

上野, 恵美

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中西, 郁夫

× 中西, 郁夫

WEKO 802879

中西, 郁夫

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松本, 謙一郎

× 松本, 謙一郎

WEKO 802880

松本, 謙一郎

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Ueno, Megumi

× Ueno, Megumi

WEKO 802881

en Ueno, Megumi

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Nakanishi, Ikuo

× Nakanishi, Ikuo

WEKO 802882

en Nakanishi, Ikuo

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Matsumoto, Kenichiro

× Matsumoto, Kenichiro

WEKO 802883

en Matsumoto, Kenichiro

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抄録
内容記述タイプ Abstract
内容記述 It has been reported that an electron paramagnetic resonance (EPR) signal of a nitroxyl radical redox probe in water could be decreased independently with a hydrogen-donor, such as GSH or NAD(P)H, when a relatively high dose X-ray or Carbon beam was irradiated to the sample solution. Since this hydrogen-donor-independent reduction of nitroxyl radial was partly suppressed by catalase, the generation of hydrogen peroxide (H2O2) is related to this reaction. However, the detailed mechanism of this hydrogen-donor-independent reduction of a nitroxyl radical was in progress.
Reaction mixtures containing 0.1 mM of a nitroxyl redox probe, TEMPOL or Carbamoyl-PROXYL, were prepared using water solvents containing several different concentrations (1.0 mM‒9.8 M) of H2O2. The reaction mixture was irradiated by 12000 μW/cm2 UVB for 2 min, and the reduction of the EPR signal of the nitorxyl radical was measured. An aliquot (2 µL) of 200 mM K3[Fe(CN)6] solution was added to 200 µL of the reaction mixture, and the time course of the EPR signal of the nitorxyl radical was measured.
The UV induced EPR signal loss of the nitorxyl radical was increased with H2O2 higher than 10 mM. The EPR signal of nitorxyl radicals were decreased in the solution containing quite high concentration (M level) of H2O2, when K3[Fe(CN)6] was added to the reaction mixture. Oxidative stimulation by UVB irradiation or addition of K3[Fe(CN)6] could cause nitorxyl radical reduction in a high concentration H2O2 environment. This result suggests that ionizing radiations could prepare a high concentration H2O2 environment, which may be localized.
会議概要(会議名, 開催地, 会期, 主催者等)
内容記述タイプ Other
内容記述 日本放射線影響学会第62回大会
発表年月日
日付 2019-11-15
日付タイプ Issued
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