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Role of Endoplasmic Reticulum and Mitochondrion in Proton Microbeam Radiation-Induced Bystander Effect
https://repo.qst.go.jp/records/77427
https://repo.qst.go.jp/records/7742703c540bb-5182-4e64-b98d-d2b275fe970b
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-09-17 | |||||
| タイトル | ||||||
| タイトル | Role of Endoplasmic Reticulum and Mitochondrion in Proton Microbeam Radiation-Induced Bystander Effect | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Dong, Chen
× Dong, Chen× Tu, Wenzhi× He, Mingyuan× Fu, Jiamei× Kobayashi, Alisa× Konishi, Teruaki× Shao, Chunlin× Alisa, Kobayashi× Teruaki, Konishi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | It is well known that mitochondria and endoplasmic reticulum (ER) plays important roles in radiation responses, but their functions in radiation-induced bystander effect (RIBE) are largely unknown. This study found that, when a small portion of cells in the population of human lung fibroblast MRC-5 cells were precisely irradiated through either nuclear or cytoplasm with counted microbeam protons, the yield of micronucleus (MN) in the nonirradiated cells neighboring to irradiated cells was increased and the levels of intracellular reactive oxygen species (ROS) in the whole population were also significantly increased after irradiation. The mito/ER-tracker assay illustrates that the mitochondria was obviously activated after nuclear irradiation and the mass of ER approached to a higher level after cytoplasmic irradiation. Moreover, the above radiation induced ROS was diminished by rotenone, an inhibitor of mitochondria activation, but it was not influenced by siRNA interference of BiP, an ER regulation protein. For nuclear irradiation, rotenone enhanced radiation-induced ER expression, and BiP siRNA eliminated radiation-induced activation of mitochondria. However, these phenomena were not observed for cytoplasmic irradiation. Bystander MN was reduced by rotenone but enhanced by BiP siRNA. When the cells were treated with both rotenone and BiP siRNA, the MN yield was reduced for nuclear irradiation but was enhanced for cytoplasmic irradiation. Our results suggest that the cell organelles of mitochondria and ER have different roles in RIBE with respect to nuclear and cytoplasmic irradiations, and the function of ER is a prerequisite for mitochondrial activation. | |||||
| 書誌情報 |
Radiation research 巻 193, 号 1, p. 63-72, 発行日 2020-01 |
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| 出版者 | ||||||
| 出版者 | Radiation Research Society | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1667/RR15469.1 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1667/RR15469.1 | |||||
