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Detection of Radical Species after Plateau Carbon-Ion Irradiation to Lipid Molecules
https://repo.qst.go.jp/records/76617
https://repo.qst.go.jp/records/76617719195b2-cc60-4d13-be90-b6b061ff600d
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-05-20 | |||||
| タイトル | ||||||
| タイトル | Detection of Radical Species after Plateau Carbon-Ion Irradiation to Lipid Molecules | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakanishi, Ikuo
× Nakanishi, Ikuo× Shoji, Yoshimi× Ueno, Megumi× Emiko, Sekine-Suzuki× Matsuoka, Yuta× Shimokawa, Takashi× Ken-ichi, Yamada× Ken-ichiro, Matsumoto× Nakanishi, Ikuo× Shoji, Yoshimi× Ueno, Megumi× Sekine, Emiko× Shimokawa, Takashi× Matsumoto, Kenichiro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Carbon-ion (C-ion) beams are known to exhibit a sharp maximum of the energy deposition, called as a Bragg peak, at a certain penetration depth. Thus, the cancer cells are precisely killed by adjusting the Bragg peak to a tumor. However, the dose in the plateau region of the C-ion beams before the Bragg peak is not necessarily zero. Thus, the quantitative evaluation of changes in the redox state induced by the plateau C-ion beams is considerable importance to eliminate the adverse effects of radiation therapy on normal tissues adjacent to the tumor. We have recently demonstrated that isopropyl myristate (IPM), one of lipid molecules, is more susceptible to the plateau C-ion beams than to X-rays using 2,2-diphenyl-1-picrylhydrazyl radical used as a redox probe. In this study, the effect of the plateau C-ion beams on lipid molecules was further investigated by 2,2,6-trimethyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)-6-pentylpiperidine-1-oxyl (NBD-Pen), a highly selective fluorescence probe for lipid radicals with high sensitivity. NBD-Pen reacts with the lipid radicals generated by the oxidation of lipid molecules to form highly fluorescent radical adducts. IPM solutions of NBD-Pen (5.0 µM) were irradiated by the plateau C-ion beams (290 MeV, 13 keV/µm) using the Heavy Ion Medical Accelerator in Chiba (HIMAC) at room temperature. An increase in fluorescence intensity at 509 nm was observed for the IPM solutions of NBD-Pen after the C-ion irradiation (5, 10, 15, 20, and 30 Gy) on a Shimadzu RF-5300PC spectrofluorophotometer. This indicates that radical species were generated by the C-ion irradiation to IPM. The fluorescence intensity increased with an increase in the radiation dose and reached its maximum value around at 10 Gy. The effect of the plateau C-ion beams on the lipid oxidation will be discussed as compared to that of X-rays based on the results obtained in this study. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 16th International Congress of Radiation Research (ICRR 2019) | |||||
| 発表年月日 | ||||||
| 日付 | 2019-08-26 | |||||
| 日付タイプ | Issued | |||||
