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Hypoxia and proton microbeams: Role of gap junction communication in inducing bystander response on human lung cancer and normal human lung cells
https://repo.qst.go.jp/records/76614
https://repo.qst.go.jp/records/766142476aedd-2093-442a-b607-6dd74e9a39a0
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-08-30 | |||||
| タイトル | ||||||
| タイトル | Hypoxia and proton microbeams: Role of gap junction communication in inducing bystander response on human lung cancer and normal human lung cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Autsavapromporn, Narongchai
× Autsavapromporn, Narongchai× Liu, Cuihua× Kobayashi, Alisa× Ahbrizal Farizal Tengku Ahmad, Tengku× Konishi, Teruaki× Liu, Cuihua× Kobayashi, Alisa× Konishi, Teruaki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Hypoxia and proton-induced bystander response is an important contributory factor to tumor resistance and normal tissue damage, subsequently lead to treatment failure and affect the quality of life. Previously, we have shown that gap-junction intercellular communication (GJIC) mediates the propagation of protective or stress responses from irradiated to bystander cells. Aim: The aim of this study is to investigate the role of GJIC and hypoxia-induced bystander response in lung cancer and normal human lung cells under normoxic and hypoxic conditions exposed to proton microbeams. Methods: Human non-small cell lung adenocarcinoma cells (A549) and normal lung fibroblasts (WI-38) were incubated either in normoxic or hypoxic conditions for 2 days. Prior to proton microbeam irradiation (LET 11 keV/m), the culture media was removed and irradiation is carried out at mean absorbed doses of 2 Gy by which 0.04-0.15% of the cells were directly targeted by protons. Following 6 hour incubation under normoxic and hypoxic conditions, the cells were harvested and assayed for micronucleus formation and chromosomal aberration. Results: The results showed that the levels of DNA damage and chromosomal aberrations in bystander hypoxia A549 cells decreased compared with cells under normoxia. On the other hand, the comparison between A549 cancer and WI-38 normal cells under hypoxia showed increased of DNA damage and dicentric chromosomes in A549 cells, but not in WI-38 cells. Interestingly, downregulation of GJIC decreased toxicity in A549 cells but increased it in WI-38 cells. This result indicates that GJIC plays a role in transmitting of protective or stress signaling between irradiated to bystander cells. Therefore, GJIC protects WI-38 cells from toxicity while enhancing it in A549 cells. Conclusion: These results show for the first time that the hypoxia-induced bystander responses is differs between cancer and normal cells, and GJIC play a role in influencing the radiosensitivity of hypoxic cancer cells against radiation. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 16th International Congress of Radiation Research | |||||
| 発表年月日 | ||||||
| 日付 | 2019-08-26 | |||||
| 日付タイプ | Issued | |||||
